3so2

<StructureSection load='3so2' size='340' side='right' caption='[[3so2]], [[Resolution|resolution]] 1.64&Aring;' scene=''>

<table><tr><td colspan='2'>[[3so2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_variabilis Chlorella variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SO2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SO2 FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CHLNCDRAFT_27939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=554065 Chlorella variabilis])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3so2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3so2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3so2 RCSB], [http://www.ebi.ac.uk/pdbsum/3so2 PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

Deoxyuridine triphosphatase (dUTPase) is a ubiquitous enzyme that has been widely studied owing to its function and evolutionary significance. The gene coding for the dUTPase from the Chlorella alga was codon-optimized and synthesized. The synthetic gene was expressed in Escherichia coli and recombinant core Chlorella dUTPase (chdUTPase) was purified. Crystallization of chdUTPase was performed by the repetitive hanging-drop vapor-diffusion method at 298 K with ammonium sulfate as the precipitant. In the presence of 2'-deoxyuridine-5'-[(alpha,beta)-imido]triphosphate and magnesium, the enzyme produced die-shaped hexagonal R3 crystals with unit-cell parameters a = b = 66.9, c = 93.6 A, gamma = 120 degrees . X-ray diffraction data for chdUTPase were collected to 1.6 A resolution. The crystallization of chdUTPase with manganese resulted in very fragile clusters of needles.

 

Crystallization of Chlorella deoxyuridine triphosphatase.,Badalucco L, Poudel I, Yamanishi M, Natarajan C, Moriyama H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1599-602. , Epub 2011 Nov 26. PMID:22139176<ref>PMID:22139176</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<references/>

[[Category: DUTP diphosphatase]]

[[Category: Badalucco, L]]

[[Category: Moriyama, H]]

[[Category: Natarajan, c]]

[[Category: Poudel, I]]

[[Category: Yamanishi, M]]

[[Category: Hydrolase]]