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3s0t

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==Crystal structure of the CofA Type IV pilin subunit from enterotoxigenic E. coli==
==Crystal structure of the CofA Type IV pilin subunit from enterotoxigenic E. coli==
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<StructureSection load='3s0t' size='340' side='right' caption='[[3s0t]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
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<StructureSection load='3s0t' size='340' side='right'caption='[[3s0t]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3s0t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S0T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3s0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S0T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.26&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s0t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s0t RCSB], [http://www.ebi.ac.uk/pdbsum/3s0t PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s0t OCA], [https://pdbe.org/3s0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s0t RCSB], [https://www.ebi.ac.uk/pdbsum/3s0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s0t ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q59393_ECOLX Q59393_ECOLX]
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The Type IV pili are helical filaments found on many Gram-negative pathogenic bacteria, with multiple diverse roles in pathogenesis, including microcolony formation, adhesion and twitching motility. Many pathogenic enterotoxigenic Escherichia coli (ETEC) isolates express one of two Type IV pili belonging to the Type IVb subclass: CFA/III or Longus. Here we show a direct correlation between CFA/III expression and ETEC aggregation, suggesting that these pili, like the Vibrio cholerae toxin coregulated pili (TCP), mediate microcolony formation. We report a 1.26 A resolution crystal structure of CofA, the major pilin subunit from CFA/III. CofA is very similar in structure to V. cholerae TcpA, but possesses a 10-amino acid insertion that replaces part of the alpha2 helix with an irregular loop containing a 3(10) helix. Homology modeling suggests a very similar structure for the Longus LngA pilin. A model for the CFA/III pilus filament was generated using the TCP electron microscopy reconstruction as a template. The unique 3(10)-helix insert fits perfectly within the gap between CofA globular domains. This insert, together with differences in surface-exposed residues, produces a filament that is smoother and more negatively charged than TCP. To explore the specificity of the Type IV pilus assembly apparatus, CofA was expressed heterologously in V. cholerae by replacing the tcpA gene with that of cofA within the tcp operon. Although CofA was synthesized and processed by V. cholerae, no CFA/III filaments were detected, suggesting that the components of the Type IVb pilus assembly system are highly specific to their pilin substrates.
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Structural characterization of CFA/III and longus type IVB pili from enterotoxigenic Escherichia coli.,Kolappan S, Roos J, Yuen A, Pierce OM, Craig L J Bacteriol. 2012 Mar 23. PMID:22447901<ref>PMID:22447901</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Pilin|Pilin]]
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*[[Pilin 3D structures|Pilin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Craig, L]]
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[[Category: Large Structures]]
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[[Category: Kolappan, S]]
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[[Category: Craig L]]
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[[Category: Bacterial surface]]
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[[Category: Kolappan S]]
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[[Category: Cell adhesion]]
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[[Category: Colonization factor]]
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[[Category: Fiber-forming protein]]
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[[Category: Pilus]]
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Current revision

Crystal structure of the CofA Type IV pilin subunit from enterotoxigenic E. coli

PDB ID 3s0t

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