2gqq

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[[Image:2gqq.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)==
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|PDB= 2gqq |SIZE=350|CAPTION= <scene name='initialview01'>2gqq</scene>, resolution 3.2&Aring;
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<StructureSection load='2gqq' size='340' side='right'caption='[[2gqq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2gqq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE= lrp, alsB, ihb, livR, oppI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gqq OCA], [https://pdbe.org/2gqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gqq RCSB], [https://www.ebi.ac.uk/pdbsum/2gqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gqq ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LRP_ECOLI LRP_ECOLI] Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/2gqq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gqq ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)'''
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==See Also==
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of Escherichia coli leucine-responsive regulatory protein (Lrp) co-crystallized with a short duplex oligodeoxynucleotide reveals a novel quaternary assembly in which the protein octamer forms an open, linear array of four dimers. In contrast, structures of the Lrp homologs LrpA, LrpC and AsnC crystallized in the absence of DNA show that these proteins instead form highly symmetrical octamers in which the four dimers form a closed ring. Although the DNA is disordered within the Lrp crystal, comparative analyses suggest that the observed differences in quaternary state may arise from DNA interactions during crystallization. Interconversion of these conformations, possibly in response to DNA or leucine binding, provides an underlying mechanism to alter the relative spatial orientation of the DNA-binding domains. Breaking of the closed octamer symmetry may be a common essential step in the formation of active DNA complexes by all members of the Lrp/AsnC family of transcriptional regulatory proteins.
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==About this Structure==
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2GQQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQQ OCA].
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==Reference==
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Structure of the Escherichia coli leucine-responsive regulatory protein Lrp reveals a novel octameric assembly., de los Rios S, Perona JJ, J Mol Biol. 2007 Mar 9;366(5):1589-602. Epub 2006 Dec 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17223133 17223133]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Perona, J J.]]
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[[Category: Perona JJ]]
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[[Category: Rios, S de los.]]
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[[Category: De los Rios S]]
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[[Category: helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:07:52 2008''
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Current revision

Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)

PDB ID 2gqq

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