3tj1

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==Crystal Structure of RNA Polymerase I Transcription Initiation Factor Rrn3==
==Crystal Structure of RNA Polymerase I Transcription Initiation Factor Rrn3==
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<StructureSection load='3tj1' size='340' side='right' caption='[[3tj1]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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<StructureSection load='3tj1' size='340' side='right'caption='[[3tj1]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tj1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJ1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tj1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJ1 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RRN3, YKL125W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tj1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tj1 RCSB], [http://www.ebi.ac.uk/pdbsum/3tj1 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tj1 OCA], [https://pdbe.org/3tj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tj1 RCSB], [https://www.ebi.ac.uk/pdbsum/3tj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tj1 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RRN3_YEAST RRN3_YEAST] Required for efficient transcription initiation by RNA polymerase I. Interacts with Pol I in the absence of template DNA and stimulates recruitment of Pol I, but does not remain as part of stable preinitiation complex.
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Cell growth is regulated during RNA polymerase (Pol) I transcription initiation by the conserved factor Rrn3/TIF-IA in yeast/humans. Here we provide a structure-function analysis of Rrn3 based on a combination of structural biology with in vivo and in vitro functional assays. The Rrn3 crystal structure reveals a unique HEAT repeat fold and a surface serine patch. Phosphorylation of this patch represses human Pol I transcription, and a phospho-mimetic patch mutation prevents Rrn3 binding to Pol I in vitro and reduces cell growth and Pol I gene occupancy in vivo. Cross-linking indicates that Rrn3 binds Pol I between its subcomplexes, AC40/19 and A14/43, which faces the serine patch. The corresponding region of Pol II binds the Mediator head that cooperates with transcription factor (TF) IIB. Consistent with this, the Rrn3-binding factor Rrn7 is predicted to be a TFIIB homolog. This reveals the molecular basis of Rrn3-regulated Pol I initiation and cell growth, and indicates a general architecture of eukaryotic transcription initiation complexes.
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Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth.,Blattner C, Jennebach S, Herzog F, Mayer A, Cheung AC, Witte G, Lorenzen K, Hopfner KP, Heck AJ, Aebersold R, Cramer P Genes Dev. 2011 Sep 22. PMID:21940764<ref>PMID:21940764</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Large Structures]]
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[[Category: Aebersold, R]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Blattner, C]]
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[[Category: Aebersold R]]
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[[Category: Cheung, A C.M]]
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[[Category: Blattner C]]
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[[Category: Cramer, P]]
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[[Category: Cheung ACM]]
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[[Category: Heck, A J.R]]
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[[Category: Cramer P]]
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[[Category: Herzog, F]]
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[[Category: Heck AJR]]
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[[Category: Hopfner, K-P]]
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[[Category: Herzog F]]
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[[Category: Jennebach, S]]
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[[Category: Hopfner K-P]]
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[[Category: Lorenzen, K]]
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[[Category: Jennebach S]]
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[[Category: Mayer, A]]
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[[Category: Lorenzen K]]
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[[Category: Witte, G]]
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[[Category: Mayer A]]
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[[Category: Heat repeat]]
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[[Category: Witte G]]
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[[Category: Nucleus]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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Current revision

Crystal Structure of RNA Polymerase I Transcription Initiation Factor Rrn3

PDB ID 3tj1

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