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4f6l

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==Crystal structure of Aureusimine biosynthetic cluster reductase domain==
==Crystal structure of Aureusimine biosynthetic cluster reductase domain==
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<StructureSection load='4f6l' size='340' side='right' caption='[[4f6l]], [[Resolution|resolution]] 3.86&Aring;' scene=''>
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<StructureSection load='4f6l' size='340' side='right'caption='[[4f6l]], [[Resolution|resolution]] 3.86&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4f6l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F6L FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4f6l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F6L FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f6c|4f6c]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.856&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV0179 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f6l OCA], [https://pdbe.org/4f6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f6l RCSB], [https://www.ebi.ac.uk/pdbsum/4f6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f6l ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f6l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f6l RCSB], [http://www.ebi.ac.uk/pdbsum/4f6l PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0H3JX00_STAAM A0A0H3JX00_STAAM]
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Through a number of strategies nonribosomal peptide assembly lines give rise to a metabolic diversity not possible by ribosomal synthesis. One distinction within nonribosomal assembly is that products are elaborated on an enzyme-tethered substrate, and their release is enzyme catalysed. Reductive release by NAD(P)H-dependent catalysts is one observed nonribosomal termination and release strategy. Here we probed the selectivity of a terminal reductase domain by using a full-length heterologously expressed nonribosomal peptide synthetase for the dipeptide aureusimine and were able to generate 17 new analogues. Further, we generated an X-ray structure of aureusimine terminal reductase to gain insight into the structural details associated with this enzymatic domain.
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Heterologous expression and structural characterisation of a pyrazinone natural product assembly line.,Wyatt MA, Mok MC, Junop M, Magarvey NA Chembiochem. 2012 Nov 5;13(16):2408-15. doi: 10.1002/cbic.201200340. Epub 2012, Oct 15. PMID:23070851<ref>PMID:23070851</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Staphylococcus aureus subsp. aureus mu50]]
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[[Category: Large Structures]]
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[[Category: Junop, M]]
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[[Category: Staphylococcus aureus subsp. aureus Mu50]]
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[[Category: Mok, M]]
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[[Category: Junop M]]
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[[Category: Oxidoreductase]]
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[[Category: Mok M]]
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[[Category: Thioester reductase]]
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Current revision

Crystal structure of Aureusimine biosynthetic cluster reductase domain

PDB ID 4f6l

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