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4g9n

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==Crystal structure of the Rhizoctonia solani agglutinin in complex with N'-acetyl-galactosamine==
==Crystal structure of the Rhizoctonia solani agglutinin in complex with N'-acetyl-galactosamine==
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<StructureSection load='4g9n' size='340' side='right' caption='[[4g9n]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='4g9n' size='340' side='right'caption='[[4g9n]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4g9n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizoctonia_solani Rhizoctonia solani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G9N FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4g9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizoctonia_solani Rhizoctonia solani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G9N FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g9m|4g9m]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g9n RCSB], [http://www.ebi.ac.uk/pdbsum/4g9n PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9n OCA], [https://pdbe.org/4g9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g9n RCSB], [https://www.ebi.ac.uk/pdbsum/4g9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g9n ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/L8WGI4_THACA L8WGI4_THACA]
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Rhizoctonia solani agglutinin (RSA) is a 15.5-kDa lectin accumulated in the mycelium and sclerotia of the soil born plant pathogenic fungus R. solani. Although it is considered to serve as a storage protein and is implicated in fungal insecticidal activity, its physiological role remains unclear as a result of a lack of any structure/function relationship information. Glycan arrays showed that RSA displays high selectivity towards terminal nonreducing N-acetylgalactosamine residues. We determined the amino acid sequence of RSA and also determined the crystal structures of the free form and the RSA-N-acetylgalactosamine complex at 1.6 and 2.2 A resolution, respectively. RSA is a homodimer comprised of two monomers adopting the beta-trefoil fold. Each monomer accommodates two different carbohydrate-binding sites in an asymmetric way. Despite RSA topology similarities with R-type lectins, the two-monomer assembly involves an N-terminal swap, thus creating a dimer association novel to R-type lectins. Structural characterization of the two carbohydrate-binding sites offers insights on the structural determinants of the RSA carbohydrate specificity. DATABASE: Structural data have been deposited in the Protein Data Bank database under accession numbers 4G9M and 4G9N. STRUCTURED DIGITAL ABSTRACT: RSA and RSA bind by x-ray crystallography (View interaction).
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Structural analysis of the Rhizoctonia solani agglutinin reveals a domain-swapping dimeric assembly.,Skamnaki VT, Peumans WJ, Kantsadi AL, Cubeta MA, Plas K, Pakala S, Zographos SE, Smagghe G, Nierman WC, Van Damme EJ, Leonidas DD FEBS J. 2013 Apr;280(8):1750-63. doi: 10.1111/febs.12190. Epub 2013 Mar 7. PMID:23402398<ref>PMID:23402398</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Rhizoctonia solani]]
[[Category: Rhizoctonia solani]]
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[[Category: Kantsadi, A L]]
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[[Category: Kantsadi AL]]
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[[Category: Leonidas, D D]]
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[[Category: Leonidas DD]]
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[[Category: Skamnaki, V T]]
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[[Category: Skamnaki VT]]
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[[Category: Carbohydrate-binding specificity]]
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[[Category: Lectin]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of the Rhizoctonia solani agglutinin in complex with N'-acetyl-galactosamine

PDB ID 4g9n

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