4cos

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==Crystal structure of the PHD-Bromo-PWWP cassette of human PRKCBP1==
==Crystal structure of the PHD-Bromo-PWWP cassette of human PRKCBP1==
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<StructureSection load='4cos' size='340' side='right' caption='[[4cos]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
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<StructureSection load='4cos' size='340' side='right'caption='[[4cos]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4cos]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4cos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4COS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cos OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cos RCSB], [http://www.ebi.ac.uk/pdbsum/4cos PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cos OCA], [https://pdbe.org/4cos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cos RCSB], [https://www.ebi.ac.uk/pdbsum/4cos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cos ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PKCB1_HUMAN PKCB1_HUMAN]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Elucidation of interactions involving DNA and histone post-translational-modifications (PTMs) is essential for providing insights into complex biological functions. Reader assemblies connected by flexible linkages facilitate avidity and increase affinity; however, little is known about the contribution to the recognition process of multiple PTMs because of rigidity in the absence of conformational flexibility. Here, we resolve the crystal structure of the triple reader module (PHD-BRD-PWWP) of ZMYND8, which forms a stable unit capable of simultaneously recognizing multiple histone PTMs while presenting a charged platform for association with DNA. Single domain disruptions destroy the functional network of interactions initiated by ZMYND8, impairing recruitment to sites of DNA damage. Our data establish a proof of principle that rigidity can be compensated by concomitant DNA and histone PTM interactions, maintaining multivalent engagement of transient chromatin states. Thus, our findings demonstrate an important role for rigid multivalent reader modules in nucleosome binding and chromatin function.
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Multivalent Histone and DNA Engagement by a PHD/BRD/PWWP Triple Reader Cassette Recruits ZMYND8 to K14ac-Rich Chromatin.,Savitsky P, Krojer T, Fujisawa T, Lambert JP, Picaud S, Wang CY, Shanle EK, Krajewski K, Friedrichsen H, Kanapin A, Goding C, Schapira M, Samsonova A, Strahl BD, Gingras AC, Filippakopoulos P Cell Rep. 2016 Dec 6;17(10):2724-2737. doi: 10.1016/j.celrep.2016.11.014. PMID:27926874<ref>PMID:27926874</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4cos" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Arrowsmith, C H]]
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[[Category: Large Structures]]
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[[Category: Bountra, C]]
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[[Category: Arrowsmith CH]]
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[[Category: Cooper, C D.O]]
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[[Category: Bountra C]]
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[[Category: Delft, F Von]]
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[[Category: Cooper CDO]]
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[[Category: Edwards, A]]
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[[Category: Edwards A]]
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[[Category: Filippakopoulos, P]]
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[[Category: Filippakopoulos P]]
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[[Category: Krojer, T]]
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[[Category: Krojer T]]
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[[Category: Newman, J A]]
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[[Category: Newman JA]]
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[[Category: Savitsky, P]]
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[[Category: Savitsky P]]
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[[Category: Bromo domain]]
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[[Category: Von Delft F]]
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[[Category: Phd domain]]
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[[Category: Pwwp domain]]
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[[Category: Transcription]]
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Current revision

Crystal structure of the PHD-Bromo-PWWP cassette of human PRKCBP1

PDB ID 4cos

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