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| | ==Crystal structure of Canavalia boliviana lectin in complex with Man1-4Man-OMe== | | ==Crystal structure of Canavalia boliviana lectin in complex with Man1-4Man-OMe== |
| - | <StructureSection load='4k1z' size='340' side='right' caption='[[4k1z]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4k1z' size='340' side='right'caption='[[4k1z]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4k1z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4k1z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K1Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k1y|4k1y]], [[4k20|4k20]], [[4k21|4k21]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1z RCSB], [http://www.ebi.ac.uk/pdbsum/4k1z PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1z OCA], [https://pdbe.org/4k1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k1z RCSB], [https://www.ebi.ac.uk/pdbsum/4k1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref> |
| - | Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(alpha1-3)Man(alpha1-O)Me, Man(alpha1-4)Man(alpha1-O)Me and 5-bromo-4-chloro-3-indolyl-alpha-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.
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| - | Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides.,Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454<ref>PMID:24865454</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Canavalia boliviana]] | | [[Category: Canavalia boliviana]] |
| - | [[Category: Bezerra, G A]] | + | [[Category: Large Structures]] |
| - | [[Category: Cavada, B S]] | + | [[Category: Bezerra GA]] |
| - | [[Category: Delatorre, P]] | + | [[Category: Cavada BS]] |
| - | [[Category: Gruber, K]] | + | [[Category: Delatorre P]] |
| - | [[Category: Moura, T R]] | + | [[Category: Gruber K]] |
| - | [[Category: Rocha, B A.M]] | + | [[Category: Moura TR]] |
| - | [[Category: Viertlmayr, R]] | + | [[Category: Rocha BAM]] |
| - | [[Category: Binding site]]
| + | [[Category: Viertlmayr R]] |
| - | [[Category: Carbohydrate binding]]
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| - | [[Category: Dimannoside]]
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| - | [[Category: Diocleinae lectin]]
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| - | [[Category: Jelly roll]]
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| - | [[Category: Mannose binding protein]]
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| - | [[Category: Oligomerization]]
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| Structural highlights
4k1z is a 4 chain structure with sequence from Canavalia boliviana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.3Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
LECA_CANBL D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).[1] [2]
References
- ↑ Figueiredo JG, da Silveira Bitencourt F, Beserra IG, Teixeira CS, Luz PB, Bezerra EH, Mota MR, Assreuy AM, de Queiroz Cunha F, Cavada BS, de Alencar NM. Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice. Naunyn Schmiedebergs Arch Pharmacol. 2009 Nov;380(5):407-14. doi: , 10.1007/s00210-009-0448-2. Epub 2009 Aug 25. PMID:19705102 doi:http://dx.doi.org/10.1007/s00210-009-0448-2
- ↑ Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS. Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides. PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454 doi:http://dx.doi.org/10.1371/journal.pone.0097015
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