2h6t

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[[Image:2h6t.jpg|left|200px]]
 
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{{Structure
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==Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A==
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|PDB= 2h6t |SIZE=350|CAPTION= <scene name='initialview01'>2h6t</scene>, resolution 1.900&Aring;
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<StructureSection load='2h6t' size='340' side='right'caption='[[2h6t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=IHN:3-HYDROXY-4-ISOBUTYL-4-[AMINOCARBONYLETHYL(AMINO CARBONYL-2-HYDROXY-5-METHYLHEXYL)TRI(AMINOCARBONYL ISOBUTYL)]BUTANOIC ACID'>IHN</scene>
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<table><tr><td colspan='2'>[[2h6t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans] and [https://en.wikipedia.org/wiki/Streptomyces_argenteolus_subsp._toyonakensis Streptomyces argenteolus subsp. toyonakensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H6T FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Candidapepsin Candidapepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.24 3.4.23.24]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= SAP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=STA:STATINE'>STA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6t OCA], [https://pdbe.org/2h6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h6t RCSB], [https://www.ebi.ac.uk/pdbsum/2h6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h6t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CARP3_CANAL CARP3_CANAL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/2h6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h6t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The family of secreted aspartic proteinases (Sap) encoded by 10 SAP genes is an important virulence factor during Candida albicans (C. albicans) infections. Antagonists to Saps could be envisioned to help prevent or treat candidosis in immunocompromised patients. The knowledge of several Sap structures is crucial for inhibitor design; only the structure of Sap2 is known. We report the 1.9 and 2.2 A resolution X-ray crystal structures of Sap3 in a stable complex with pepstatin A and in the absence of an inhibitor, shedding further light on the enzyme inhibitor binding. Inhibitor binding causes active site closure by the movement of a flap segment. Comparison of the structures of Sap3 and Sap2 identifies elements responsible for the specificity of each isoenzyme.
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'''Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A'''
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The crystal structure of the secreted aspartic proteinase 3 from Candida albicans and its complex with pepstatin A.,Borelli C, Ruge E, Schaller M, Monod M, Korting HC, Huber R, Maskos K Proteins. 2007 Aug 15;68(3):738-48. PMID:17510964<ref>PMID:17510964</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2h6t" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The family of secreted aspartic proteinases (Sap) encoded by 10 SAP genes is an important virulence factor during Candida albicans (C. albicans) infections. Antagonists to Saps could be envisioned to help prevent or treat candidosis in immunocompromised patients. The knowledge of several Sap structures is crucial for inhibitor design; only the structure of Sap2 is known. We report the 1.9 and 2.2 A resolution X-ray crystal structures of Sap3 in a stable complex with pepstatin A and in the absence of an inhibitor, shedding further light on the enzyme inhibitor binding. Inhibitor binding causes active site closure by the movement of a flap segment. Comparison of the structures of Sap3 and Sap2 identifies elements responsible for the specificity of each isoenzyme.
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*[[Pepsin|Pepsin]]
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== References ==
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==About this Structure==
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<references/>
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2H6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6T OCA].
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__TOC__
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</StructureSection>
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==Reference==
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The crystal structure of the secreted aspartic proteinase 3 from Candida albicans and its complex with pepstatin A., Borelli C, Ruge E, Schaller M, Monod M, Korting HC, Huber R, Maskos K, Proteins. 2007 Aug 15;68(3):738-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17510964 17510964]
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[[Category: Candida albicans]]
[[Category: Candida albicans]]
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[[Category: Candidapepsin]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptomyces argenteolus subsp. toyonakensis]]
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[[Category: Borelli, C.]]
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[[Category: Borelli C]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Maskos, K.]]
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[[Category: Maskos K]]
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[[Category: Ruge, E.]]
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[[Category: Ruge E]]
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[[Category: IHN]]
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[[Category: ZN]]
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[[Category: aspartic proteinase]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:20 2008''
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Current revision

Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A

PDB ID 2h6t

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