4oji

<StructureSection load='4oji' size='340' side='right' caption='[[4oji]], [[Resolution|resolution]] 2.30&Aring;' scene=''>

<table><tr><td colspan='2'>[[4oji]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OJI FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oji OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oji RCSB], [http://www.ebi.ac.uk/pdbsum/4oji PDBsum]</span></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

We present a crystal structure at 2.3-A resolution of the recently described nucleolytic ribozyme twister. The RNA adopts a previously uncharacterized compact fold based on a double-pseudoknot structure, with the active site at its center. Eight highly conserved nucleobases stabilize the core of the ribozyme through the formation of one Watson-Crick and three noncanonical base pairs, and the highly conserved adenine 3' of the scissile phosphate is bound in the major groove of an adjacent pseudoknot. A strongly conserved guanine nucleobase directs its Watson-Crick edge toward the scissile phosphate in the crystal structure, and mechanistic evidence supports a role for this guanine as either a general base or acid in a concerted, general acid-base-catalyzed cleavage reaction.

Crystal structure and mechanistic investigation of the twister ribozyme.,Liu Y, Wilson TJ, McPhee SA, Lilley DM Nat Chem Biol. 2014 Sep;10(9):739-44. doi: 10.1038/nchembio.1587. Epub 2014 Jul, 20. PMID:25038788<ref>PMID:25038788</ref>

== References ==

[[Category: Lilley, D M.J]]

[[Category: Liu, Y]]

[[Category: McPhee, S A]]

[[Category: Wilson, T J]]

[[Category: Pseudoknot]]

[[Category: Self-cleavage]]