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2hnk

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[[Image:2hnk.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans==
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|PDB= 2hnk |SIZE=350|CAPTION= <scene name='initialview01'>2hnk</scene>, resolution 2.300&Aring;
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<StructureSection load='2hnk' size='340' side='right'caption='[[2hnk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> and <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
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<table><tr><td colspan='2'>[[2hnk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HNK FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hnk OCA], [https://pdbe.org/2hnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hnk RCSB], [https://www.ebi.ac.uk/pdbsum/2hnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hnk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8F8Y3_LEPIN Q8F8Y3_LEPIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/2hnk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hnk ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans'''
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==See Also==
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*[[SAM-dependent methyltrasferase 3D structures|SAM-dependent methyltrasferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety.
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[[Category: Large Structures]]
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==About this Structure==
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2HNK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HNK OCA].
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==Reference==
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Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17561415 17561415]
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[[Category: Leptospira interrogans]]
[[Category: Leptospira interrogans]]
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[[Category: Single protein]]
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[[Category: Gong W]]
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[[Category: Gong, W.]]
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[[Category: Hou X]]
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[[Category: Hou, X.]]
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[[Category: Wei Z]]
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[[Category: Wei, Z.]]
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[[Category: PEG]]
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[[Category: SAH]]
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[[Category: SO4]]
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[[Category: modified rossman fold]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:19:16 2008''
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Current revision

Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans

PDB ID 2hnk

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