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1n9n

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Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.

Structural highlights

1n9n is a 1 chain structure with sequence from Chlamydomonas reinhardtii. The March 2015 RCSB PDB Molecule of the Month feature on Phototropin by David Goodsell is 10.2210/rcsb_pdb/mom_2015_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHOT_CHLRE Protein kinase that acts as a blue light photoreceptor (PubMed:15695460, PubMed:24285544). Required for non-photochemical quenching (NPQ), a mechanism that converts and dissipates the harmful excess absorbed light energy into heat and protect the photosynthetic apparatus from photo-oxidative damage (PubMed:27626383). Controls the energy-dependent chlorophyll fluorescence quenching (qE) activity of chlorophyll excited states by inducing the expression of the qE effector protein LHCSR3 in high light intensities (PubMed:27626383).^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Phot proteins (phototropins and homologs) are blue-light photoreceptors that control mechanical processes like phototropism, chloroplast relocation, or guard-cell opening in plants. Phot receptors consist of two flavin mononucleotide (FMN)-binding light, oxygen, or voltage (LOV) domains and a C-terminal serine/threonine kinase domain. We determined crystal structures of the LOV1 domain of Phot1 from the green alga Chlamydomonas reinhardtii in the dark and illuminated state to 1.9 A and 2.8 A resolution, respectively. The structure resembles that of LOV2 from Adiantum (Crosson, S. and K. Moffat. 2001. PROC: Natl. Acad. Sci. USA. 98:2995-3000). In the resting dark state of LOV1, the reactive Cys-57 is present in two conformations. Blue-light absorption causes formation of a proposed active signaling state that is characterized by a covalent bond between the flavin C4a and the thiol of Cys-57. There are differences around the FMN chromophore but no large overall conformational changes. Quantum chemical calculations based on the crystal structures revealed the electronic distribution in the active site during the photocycle. The results suggest trajectories for electrons, protons, and the active site cysteine and offer an interpretation of the reaction mechanism.

Crystal structures and molecular mechanism of a light-induced signaling switch: The Phot-LOV1 domain from Chlamydomonas reinhardtii.,Fedorov R, Schlichting I, Hartmann E, Domratcheva T, Fuhrmann M, Hegemann P Biophys J. 2003 Apr;84(4):2474-82. PMID:12668455^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Onodera A, Kong SG, Doi M, Shimazaki K, Christie J, Mochizuki N, Nagatani A. Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana. Plant Cell Physiol. 2005 Feb;46(2):367-74. PMID:15695460 doi:10.1093/pcp/pci037
↑ Okajima K, Aihara Y, Takayama Y, Nakajima M, Kashojiya S, Hikima T, Oroguchi T, Kobayashi A, Sekiguchi Y, Yamamoto M, Suzuki T, Nagatani A, Nakasako M, Tokutomi S. Light-induced conformational changes of LOV1 (light oxygen voltage-sensing domain 1) and LOV2 relative to the kinase domain and regulation of kinase activity in Chlamydomonas phototropin. J Biol Chem. 2014 Jan 3;289(1):413-22. PMID:24285544 doi:10.1074/jbc.M113.515403
↑ Petroutsos D, Tokutsu R, Maruyama S, Flori S, Greiner A, Magneschi L, Cusant L, Kottke T, Mittag M, Hegemann P, Finazzi G, Minagawa J. A blue-light photoreceptor mediates the feedback regulation of photosynthesis. Nature. 2016 Sep 22;537(7621):563-566. PMID:27626383 doi:10.1038/nature19358
↑ Fedorov R, Schlichting I, Hartmann E, Domratcheva T, Fuhrmann M, Hegemann P. Crystal structures and molecular mechanism of a light-induced signaling switch: The Phot-LOV1 domain from Chlamydomonas reinhardtii. Biophys J. 2003 Apr;84(4):2474-82. PMID:12668455

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n9n"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.==
-<StructureSection load='1n9n' size='340' side='right' caption='[[1n9n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='1n9n' size='340' side='right'caption='[[1n9n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1n9n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N9N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1n9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N9N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g28|1g28]], [[1jnu|1jnu]], [[1n9l|1n9l]], [[1n9o|1n9o]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n9n RCSB], [http://www.ebi.ac.uk/pdbsum/1n9n PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n9n OCA], [https://pdbe.org/1n9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n9n RCSB], [https://www.ebi.ac.uk/pdbsum/1n9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n9n ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PHOT_CHLRE PHOT_CHLRE] Protein kinase that acts as a blue light photoreceptor (PubMed:15695460, PubMed:24285544). Required for non-photochemical quenching (NPQ), a mechanism that converts and dissipates the harmful excess absorbed light energy into heat and protect the photosynthetic apparatus from photo-oxidative damage (PubMed:27626383). Controls the energy-dependent chlorophyll fluorescence quenching (qE) activity of chlorophyll excited states by inducing the expression of the qE effector protein LHCSR3 in high light intensities (PubMed:27626383).<ref>PMID:15695460</ref> <ref>PMID:24285544</ref> <ref>PMID:27626383</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/1n9n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/1n9n_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n9n ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 25: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1n9n" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 30: / Line 34: @@
 </StructureSection>
 [[Category: Chlamydomonas reinhardtii]]
-[[Category: Domratcheva, T]]
+[[Category: Large Structures]]
-[[Category: Fedorov, R]]
-[[Category: Fuhrmann, M]]
-[[Category: Hartmann, E]]
-[[Category: Hegemann, P]]
-[[Category: Schlichting, I]]
-[[Category: Electron transport]]
-[[Category: Flavin]]
 [[Category: Phototropin]]
+[[Category: RCSB PDB Molecule of the Month]]
+[[Category: Domratcheva T]]
+[[Category: Fedorov R]]
+[[Category: Fuhrmann M]]
+[[Category: Hartmann E]]
+[[Category: Hegemann P]]
+[[Category: Schlichting I]]

1n9n

From Proteopedia

Current revision

Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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