2i48

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[[Image:2i48.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with carbonic acid==
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|PDB= 2i48 |SIZE=350|CAPTION= <scene name='initialview01'>2i48</scene>, resolution 1.60&Aring;
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<StructureSection load='2i48' size='340' side='right'caption='[[2i48]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CO3:CARBONATE ION'>CO3</scene>
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<table><tr><td colspan='2'>[[2i48]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I48 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= cmpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1143 Synechocystis sp.])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i48 OCA], [https://pdbe.org/2i48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i48 RCSB], [https://www.ebi.ac.uk/pdbsum/2i48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i48 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with carbonic acid'''
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== Function ==
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[https://www.uniprot.org/uniprot/CMPA_SYNY3 CMPA_SYNY3] Part of the ABC transporter complex CmpABCD involved in bicarbonate transport. Binds bicarbonate with high affinity (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form the base of the food chain by fixing carbon and nitrogen into cellular biomass. To compensate for the low selectivity of Rubisco for CO2 over O2, cyanobacteria have developed highly efficient CO2-concentrating machinery of which the ABC transport system CmpABCD from Synechocystis PCC 6803 is one component. Here, we have described the structure of the bicarbonate-binding protein CmpA in the absence and presence of bicarbonate and carbonic acid. CmpA is highly homologous to the nitrate transport protein NrtA. CmpA binds carbonic acid at the entrance to the ligand-binding pocket, whereas bicarbonate binds in nearly an identical location compared with nitrate binding to NrtA. Unexpectedly, bicarbonate binding is accompanied by a metal ion, identified as Ca2+ via inductively coupled plasma optical emission spectrometry. The binding of bicarbonate and metal appears to be highly cooperative and suggests that CmpA may co-transport bicarbonate and calcium or that calcium acts a cofactor in bicarbonate transport.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/2i48_consurf.spt"</scriptWhenChecked>
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2I48 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I48 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The structure of a cyanobacterial bicarbonate transport protein, CmpA., Koropatkin NM, Koppenaal DW, Pakrasi HB, Smith TJ, J Biol Chem. 2007 Jan 26;282(4):2606-14. Epub 2006 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17121816 17121816]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i48 ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Synechocystis sp.]]
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__TOC__
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[[Category: Koropatkin, N M.]]
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</StructureSection>
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[[Category: Pakrasi, H B.]]
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[[Category: Large Structures]]
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[[Category: Smith, T J.]]
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[[Category: Synechocystis sp. PCC 6803]]
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[[Category: CO3]]
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[[Category: Koropatkin NM]]
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[[Category: abc transporter]]
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[[Category: Pakrasi HB]]
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[[Category: alpha-beta protein]]
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[[Category: Smith TJ]]
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[[Category: bicarbonate-binding protein]]
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[[Category: c-clamp]]
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[[Category: periplasmic solute-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:25:11 2008''
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Current revision

Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with carbonic acid

PDB ID 2i48

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