2i83

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[[Image:2i83.gif|left|200px]]
 
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{{Structure
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==hyaluronan-binding domain of CD44 in its ligand-bound form==
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|PDB= 2i83 |SIZE=350|CAPTION= <scene name='initialview01'>2i83</scene>
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<StructureSection load='2i83' size='340' side='right'caption='[[2i83]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2i83]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I83 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i83 OCA], [https://pdbe.org/2i83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i83 RCSB], [https://www.ebi.ac.uk/pdbsum/2i83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i83 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''hyaluronan-binding domain of CD44 in its ligand-bound form'''
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[https://www.uniprot.org/uniprot/CD44_HUMAN CD44_HUMAN] Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/2i83_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i83 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
CD44, a major cell surface receptor for hyaluronan (HA), contains a functional domain responsible for HA binding at its N terminus (residues 21-178). Accumulating evidence indicates that proteolytic cleavage of CD44 in its extracellular region (residues 21-268) leads to enhanced tumor cell migration and invasion. Hence, understanding the mechanisms underlying the CD44 proteolytic cleavage is important for understanding the mechanism of CD44-mediated tumor progression. Here we present the NMR structure of the HA-binding domain of CD44 in its HA-bound state. The structure is composed of the Link module (residues 32-124) and an extended lobe (residues 21-31 and 125-152). Interestingly, a comparison of its unbound and HA-bound structures revealed that rearrangement of the beta-strands in the extended lobe (residues 143-148) and disorder of the structure in the following C-terminal region (residues 153-169) occurred upon HA binding, which is consistent with the results of trypsin proteolysis studies of the CD44 HA-binding domain. The order-to-disorder transition of the C-terminal region by HA binding may be involved in the CD44-mediated cell migration.
CD44, a major cell surface receptor for hyaluronan (HA), contains a functional domain responsible for HA binding at its N terminus (residues 21-178). Accumulating evidence indicates that proteolytic cleavage of CD44 in its extracellular region (residues 21-268) leads to enhanced tumor cell migration and invasion. Hence, understanding the mechanisms underlying the CD44 proteolytic cleavage is important for understanding the mechanism of CD44-mediated tumor progression. Here we present the NMR structure of the HA-binding domain of CD44 in its HA-bound state. The structure is composed of the Link module (residues 32-124) and an extended lobe (residues 21-31 and 125-152). Interestingly, a comparison of its unbound and HA-bound structures revealed that rearrangement of the beta-strands in the extended lobe (residues 143-148) and disorder of the structure in the following C-terminal region (residues 153-169) occurred upon HA binding, which is consistent with the results of trypsin proteolysis studies of the CD44 HA-binding domain. The order-to-disorder transition of the C-terminal region by HA binding may be involved in the CD44-mediated cell migration.
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==Disease==
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Ligand-induced structural changes of the CD44 hyaluronan-binding domain revealed by NMR.,Takeda M, Ogino S, Umemoto R, Sakakura M, Kajiwara M, Sugahara KN, Hayasaka H, Miyasaka M, Terasawa H, Shimada I J Biol Chem. 2006 Dec 29;281(52):40089-95. Epub 2006 Nov 2. PMID:17085435<ref>PMID:17085435</ref>
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Known diseases associated with this structure: Blood group, Indian system OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=107269 107269]], Fertile eunuch syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=138850 138850]], Hypogonadotropic hypogonadism OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=138850 138850]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2I83 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I83 OCA].
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</div>
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<div class="pdbe-citations 2i83" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Ligand-induced structural changes of the CD44 hyaluronan-binding domain revealed by NMR., Takeda M, Ogino S, Umemoto R, Sakakura M, Kajiwara M, Sugahara KN, Hayasaka H, Miyasaka M, Terasawa H, Shimada I, J Biol Chem. 2006 Dec 29;281(52):40089-95. Epub 2006 Nov 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17085435 17085435]
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*[[CD44|CD44]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hayasaka, H.]]
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[[Category: Hayasaka H]]
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[[Category: Kajiwara, M.]]
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[[Category: Kajiwara M]]
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[[Category: Miyasaka, M.]]
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[[Category: Miyasaka M]]
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[[Category: Ogino, S.]]
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[[Category: Ogino S]]
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[[Category: Sakakura, M.]]
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[[Category: Sakakura M]]
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[[Category: Shimada, I.]]
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[[Category: Shimada I]]
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[[Category: Sugahara, K N.]]
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[[Category: Sugahara KN]]
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[[Category: Takeda, M.]]
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[[Category: Takeda M]]
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[[Category: Terasawa, H.]]
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[[Category: Terasawa H]]
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[[Category: Umemoto, R.]]
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[[Category: Umemoto R]]
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[[Category: link module]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:26:32 2008''
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Current revision

hyaluronan-binding domain of CD44 in its ligand-bound form

PDB ID 2i83

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