2iag

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[[Image:2iag.gif|left|200px]]
 
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{{Structure
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==Crystal structure of human prostacyclin synthase==
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|PDB= 2iag |SIZE=350|CAPTION= <scene name='initialview01'>2iag</scene>, resolution 2.15&Aring;
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<StructureSection load='2iag' size='340' side='right'caption='[[2iag]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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<table><tr><td colspan='2'>[[2iag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAG FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Prostaglandin-I_synthase Prostaglandin-I synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.4 5.3.99.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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|GENE= PTGIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iag OCA], [https://pdbe.org/2iag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iag RCSB], [https://www.ebi.ac.uk/pdbsum/2iag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iag ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of human prostacyclin synthase'''
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== Function ==
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[https://www.uniprot.org/uniprot/PTGIS_HUMAN PTGIS_HUMAN] Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Prostacyclin synthase (PGIS) catalyzes an isomerization of prostaglandin H(2) to prostacyclin, a potent mediator of vasodilation and anti-platelet aggregation. Here, we report the crystal structure of human PGIS at 2.15 A resolution, which represents the first three-dimensional structure of a class III cytochrome P450. While notable sequence divergence has been recognized between PGIS and other P450s, PGIS exhibits the typical triangular prism-shaped P450 fold with only moderate structural differences. The conserved acid-alcohol pair in the I helix of P450s is replaced by residues G286 and N287 in PGIS, but the distinctive disruption of the I helix and the presence of a nearby water channel remain conserved. The side-chain of N287 appears to be positioned to facilitate the endoperoxide bond cleavage, suggesting a functional conservation of this residue in O-O bond cleavage. A combination of bent I helix and tilted B' helix creates a channel extending from the heme distal pocket, which seemingly allows binding of various ligands; however, residue W282, placed in this channel at a distance of 8.4 A from the iron with its indole side-chain lying parallel with the porphyrin plane, may serve as a threshold to exclude most ligands from binding. Additionally, a long "meander" region protruding from the protein surface may impede electron transfer. Although the primary sequence of the PGIS cysteine ligand loop diverges significantly from the consensus, conserved tertiary structure and hydrogen bonding pattern are observed for this region. The substrate-binding model was constructed and the structural basis for prostacyclin biosynthesis is discussed.
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Check<jmol>
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<jmolCheckbox>
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==Disease==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iag_consurf.spt"</scriptWhenChecked>
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Known diseases associated with this structure: Hypertension, essential OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601699 601699]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2IAG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAG OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iag ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of the human prostacyclin synthase., Chiang CW, Yeh HC, Wang LH, Chan NL, J Mol Biol. 2006 Dec 1;364(3):266-74. Epub 2006 Sep 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17020766 17020766]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Prostaglandin-I synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Chan N-L]]
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[[Category: Chan, N L.]]
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[[Category: Chiang C-W]]
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[[Category: Chiang, C W.]]
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[[Category: Wang L-H]]
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[[Category: Wang, L H.]]
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[[Category: Yeh H-C]]
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[[Category: Yeh, H C.]]
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[[Category: HEM]]
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[[Category: NA]]
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[[Category: class iii cytochorme p450]]
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[[Category: cyp8a1]]
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[[Category: hemoprotein]]
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[[Category: prostacyclin synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:27:09 2008''
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Current revision

Crystal structure of human prostacyclin synthase

PDB ID 2iag

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