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| - | [[Image:2iic.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Calcium bound structure of alpha-11 giardin== |
| - | |PDB= 2iic |SIZE=350|CAPTION= <scene name='initialview01'>2iic</scene>, resolution 2.930Å
| + | <StructureSection load='2iic' size='340' side='right'caption='[[2iic]], [[Resolution|resolution]] 2.93Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | <table><tr><td colspan='2'>[[2iic]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IIC FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.93Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iic OCA], [https://pdbe.org/2iic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iic RCSB], [https://www.ebi.ac.uk/pdbsum/2iic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iic ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''Calcium bound structure of alpha-11 giardin'''
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/Q4VPP2_GIAIN Q4VPP2_GIAIN] |
| - | | + | == Evolutionary Conservation == |
| - | ==Overview== | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | Alpha-11 giardin is a member of the multi-gene alpha-giardin family in the intestinal protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium-dependent binding to membranes that contain acidic phospholipids. Several alpha giardins are highly expressed during parasite-induced diarrhea in humans. Despite being a member of a large family of proteins, little is known about the function and cellular localization of alpha-11 giardin, although giardins are often associated with the cytoskeleton. It has been shown that Giardia exhibits high levels of alpha-11 giardin mRNA transcript throughout its life cycle; however, constitutive over-expression of this protein is lethal to the parasite. Determining the three-dimensional structure of an alpha-giardin is essential to identifying functional domains shared in the alpha-giardin family. Here we report the crystal structures of the apo and Ca(2+)-bound forms of alpha-11 giardin, the first alpha giardin to be characterized structurally. Crystals of apo and Ca(2+)-bound alpha-11 giardin diffracted to 1.1 A and 2.93 A, respectively. The crystal structure of selenium-substituted apo alpha-11 giardin reveals a planar array of four tandem repeats of predominantly alpha-helical domains, reminiscent of previously determined annexin structures, making this the highest-resolution structure of an annexin to date. The apo alpha-11 giardin structure also reveals a hydrophobic core formed between repeats I/IV and II/III, a region typically hydrophilic in other annexins. Surprisingly, the Ca(2+)-bound structure contains only a single calcium ion, located in the DE loop of repeat I and coordinated differently from the two types of calcium sites observed in previous annexin structures. The apo and Ca(2+)-bound alpha-11 giardin structures assume overall similar conformations; however, Ca(2+)-bound alpha-11 giardin crystallized in a lower-symmetry space group with four molecules in the asymmetric unit. Vesicle-binding studies suggest that alpha-11 giardin, unlike most other annexins, does not bind to vesicles composed of acidic phospholipids in a calcium-dependent manner.
| + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==About this Structure== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/2iic_consurf.spt"</scriptWhenChecked> |
| - | 2IIC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IIC OCA].
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==Reference== | + | </jmolCheckbox> |
| - | Apo and calcium-bound crystal structures of Alpha-11 giardin, an unusual annexin from Giardia lamblia., Pathuri P, Nguyen ET, Svard SG, Luecke H, J Mol Biol. 2007 Apr 27;368(2):493-508. Epub 2007 Feb 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355882 17355882]
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iic ConSurf]. |
| | + | <div style="clear:both"></div> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Giardia intestinalis]] | | [[Category: Giardia intestinalis]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Luecke, H.]] | + | [[Category: Luecke H]] |
| - | [[Category: Nguyen, E T.]] | + | [[Category: Nguyen ET]] |
| - | [[Category: Pathuri, P.]] | + | [[Category: Pathuri P]] |
| - | [[Category: CA]]
| + | |
| - | [[Category: calcium-protein complex]]
| + | |
| - | [[Category: helix-turn-helix]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:29:59 2008''
| + | |
