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'''Cytokinin deshydrogenase'''([http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Wikipedia]), also called CKX, is an enzyme which '''degrades cytokinin hormones''' in plants and which is encoded by the gene '''ZmCKX1'''. The one is located in '''Zea Maize''' on the chromosome 3 (NCBI Gene ID: 542585) and belongs to a multigene family called '''V'''anillyl-'''A'''lcohol '''O'''xidase (VAO) flavoprotein family. The gene is more particularly expressed in the kernel of maize, mainly in the embryo since it may protect the embryo from too much cytokinin and permit the correct development. <ref name="Frebortova">PMID: 19912568 </ref>

Cytokine deshydrogenase are extracellular and monomeric proteins with a molecular weight of 63kDa.<ref name="Kopečnýa">PMID: 20478354 </ref><ref name="Kopečnýa">PMID: 18571199 </ref> For protein production purposes, ZmCKO1 precursor protein was truncated by deletion of 18 N-terminal amino acids to produce the expected mature enzyme. [8]

The Enzyme Classification number of CKX is EC 1.5.99.12 and this indicates that the enzyme is an oxydo reductase which acts on the CH-NH group of the donor. Consequently the reaction of CKX with its cytokinin substrate is a transfer of two electrons from the cytokinin to an acceptor which is in the case of CKX the Flavine Adenine Nucleotide (FAD) cofactor. [1]

== '''References''' ==

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