4x8d
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Ergothioneine-biosynthetic sulfoxide synthase EgtB in complex with N,N-dimethyl-histidine and gamma-glutamyl-cysteine== | |
| + | <StructureSection load='4x8d' size='340' side='right'caption='[[4x8d]], [[Resolution|resolution]] 1.98Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4x8d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_thermoresistibile_ATCC_19527 Mycolicibacterium thermoresistibile ATCC 19527]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X8D FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3GC:GAMMA-GLUTAMYLCYSTEINE'>3GC</scene>, <scene name='pdbligand=AVI:N,N-DIMETHYL-L-HISTIDINE'>AVI</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8d OCA], [https://pdbe.org/4x8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x8d RCSB], [https://www.ebi.ac.uk/pdbsum/4x8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x8d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/EGTB_MYCT3 EGTB_MYCT3] Catalyzes the oxidative sulfurization of hercynine (N-alpha,N-alpha,N-alpha-trimethyl-L-histidine) into hercynyl-gamma-L-glutamyl-L-cysteine sulfoxide, a step in the biosynthesis pathway of ergothioneine.<ref>PMID:25597398</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The non-heme iron enzyme EgtB catalyzes O2 -dependent CS bond formation between gamma-glutamyl cysteine and N-alpha-trimethyl histidine as the central step in ergothioneine biosynthesis. Both, the catalytic activity and the architecture of EgtB are distinct from known sulfur transferases or thiol dioxygenases. The crystal structure of EgtB from Mycobacterium thermoresistibile in complex with gamma-glutamyl cysteine and N-alpha-trimethyl histidine reveals that the two substrates and three histidine residues serve as ligands in an octahedral iron binding site. This active site geometry is consistent with a catalytic mechanism in which CS bond formation is initiated by an iron(III)-complexed thiyl radical attacking the imidazole ring of N-alpha-trimethyl histidine. | ||
| - | + | Structure of the Sulfoxide Synthase EgtB from the Ergothioneine Biosynthetic Pathway.,Goncharenko KV, Vit A, Blankenfeldt W, Seebeck FP Angew Chem Int Ed Engl. 2015 Jan 16. doi: 10.1002/anie.201410045. PMID:25597398<ref>PMID:25597398</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4x8d" style="background-color:#fffaf0;"></div> |
| - | [[Category: Blankenfeldt | + | == References == |
| - | [[Category: Goncharenko | + | <references/> |
| - | [[Category: Vit | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycolicibacterium thermoresistibile ATCC 19527]] | ||
| + | [[Category: Blankenfeldt W]] | ||
| + | [[Category: Goncharenko KV]] | ||
| + | [[Category: Seebeck FP]] | ||
| + | [[Category: Vit A]] | ||
Current revision
Ergothioneine-biosynthetic sulfoxide synthase EgtB in complex with N,N-dimethyl-histidine and gamma-glutamyl-cysteine
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