4rwd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (03:32, 21 November 2024) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
 +
==XFEL structure of the human delta opioid receptor in complex with a bifunctional peptide==
==XFEL structure of the human delta opioid receptor in complex with a bifunctional peptide==
-
<StructureSection load='4rwd' size='340' side='right' caption='[[4rwd]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+
<StructureSection load='4rwd' size='340' side='right'caption='[[4rwd]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4rwd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RWD FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4rwd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RWD FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DI7:2,6-DIMETHYL-L-TYROSINE'>DI7</scene>, <scene name='pdbligand=DI8:(3S)-1,2,3,4-TETRAHYDROISOQUINOLINE-3-CARBOXYLIC+ACID'>DI8</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DI7:2,6-DIMETHYL-L-TYROSINE'>DI7</scene>, <scene name='pdbligand=DI8:(3S)-1,2,3,4-TETRAHYDROISOQUINOLINE-3-CARBOXYLIC+ACID'>DI8</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n6h|4n6h]], [[4ej4|4ej4]], [[4rwa|4rwa]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rwd OCA], [https://pdbe.org/4rwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rwd RCSB], [https://www.ebi.ac.uk/pdbsum/4rwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rwd ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rwd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rwd RCSB], [http://www.ebi.ac.uk/pdbsum/4rwd PDBsum]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX]] Electron-transport protein of unknown function.
+
[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.[https://www.uniprot.org/uniprot/OPRD_HUMAN OPRD_HUMAN] G-protein coupled receptor that functions as a receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:22184124</ref> <ref>PMID:7808419</ref> <ref>PMID:8201839</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Bifunctional mu- and delta-opioid receptor (OR) ligands are potential therapeutic alternatives, with diminished side effects, to alkaloid opiate analgesics. We solved the structure of human delta-OR bound to the bifunctional delta-OR antagonist and mu-OR agonist tetrapeptide H-Dmt-Tic-Phe-Phe-NH2 (DIPP-NH2) by serial femtosecond crystallography, revealing a cis-peptide bond between H-Dmt and Tic. The observed receptor-peptide interactions are critical for understanding of the pharmacological profiles of opioid peptides and for development of improved analgesics.
 +
 
 +
Structural basis for bifunctional peptide recognition at human delta-opioid receptor.,Fenalti G, Zatsepin NA, Betti C, Giguere P, Han GW, Ishchenko A, Liu W, Guillemyn K, Zhang H, James D, Wang D, Weierstall U, Spence JC, Boutet S, Messerschmidt M, Williams GJ, Gati C, Yefanov OM, White TA, Oberthuer D, Metz M, Yoon CH, Barty A, Chapman HN, Basu S, Coe J, Conrad CE, Fromme R, Fromme P, Tourwe D, Schiller PW, Roth BL, Ballet S, Katritch V, Stevens RC, Cherezov V Nat Struct Mol Biol. 2015 Mar;22(3):265-8. doi: 10.1038/nsmb.2965. Epub 2015 Feb , 16. PMID:25686086<ref>PMID:25686086</ref>
 +
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4rwd" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Ballet, S]]
+
[[Category: Escherichia coli]]
-
[[Category: Barty, A]]
+
[[Category: Homo sapiens]]
-
[[Category: Basu, S]]
+
[[Category: Large Structures]]
-
[[Category: Betti, C]]
+
[[Category: Synthetic construct]]
-
[[Category: Boutet, S]]
+
[[Category: Ballet S]]
-
[[Category: Chapman, H N]]
+
[[Category: Barty A]]
-
[[Category: Cherezov, V]]
+
[[Category: Basu S]]
-
[[Category: Coe, J]]
+
[[Category: Betti C]]
-
[[Category: Conrad, C E]]
+
[[Category: Boutet S]]
-
[[Category: Fenalti, G]]
+
[[Category: Chapman HN]]
-
[[Category: Fromme, P]]
+
[[Category: Cherezov V]]
-
[[Category: Fromme, R]]
+
[[Category: Coe J]]
-
[[Category: GPCR, GPCR Network]]
+
[[Category: Conrad CE]]
-
[[Category: Gati, C]]
+
[[Category: Fenalti G]]
-
[[Category: Giguere, P]]
+
[[Category: Fromme P]]
-
[[Category: Guillemyn, K]]
+
[[Category: Fromme R]]
-
[[Category: Han, G W]]
+
[[Category: Gati C]]
-
[[Category: Ishchenko, A]]
+
[[Category: Giguere P]]
-
[[Category: James, D]]
+
[[Category: Guillemyn K]]
-
[[Category: Katritch, V]]
+
[[Category: Han GW]]
-
[[Category: Liu, W]]
+
[[Category: Ishchenko A]]
-
[[Category: Messerschmidt, M]]
+
[[Category: James D]]
-
[[Category: Metz, M]]
+
[[Category: Katritch V]]
-
[[Category: Oberthuer, D]]
+
[[Category: Liu W]]
-
[[Category: Roth, B L]]
+
[[Category: Messerschmidt M]]
-
[[Category: Schiller, P W]]
+
[[Category: Metz M]]
-
[[Category: Spence, J C.H]]
+
[[Category: Oberthuer D]]
-
[[Category: Stevens, R C]]
+
[[Category: Roth BL]]
-
[[Category: Tourwe, D]]
+
[[Category: Schiller PW]]
-
[[Category: Wang, D]]
+
[[Category: Spence JCH]]
-
[[Category: Weierstall, U]]
+
[[Category: Stevens RC]]
-
[[Category: White, T A]]
+
[[Category: Tourwe D]]
-
[[Category: Williams, G J]]
+
[[Category: Wang D]]
-
[[Category: Yefanov, O M]]
+
[[Category: Weierstall U]]
-
[[Category: Yoon, C H]]
+
[[Category: White TA]]
-
[[Category: Zatsepin, N A]]
+
[[Category: Williams GJ]]
-
[[Category: Zhang, H]]
+
[[Category: Yefanov OM]]
-
[[Category: Bifunctional peptide]]
+
[[Category: Yoon CH]]
-
[[Category: Bril]]
+
[[Category: Zatsepin NA]]
-
[[Category: Gpcr]]
+
[[Category: Zhang H]]
-
[[Category: Gpcr network]]
+
-
[[Category: Gpcr signaling]]
+
-
[[Category: Human opioid receptor]]
+
-
[[Category: Lipidic cubic phase]]
+
-
[[Category: Membrane]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: Psi-biology]]
+
-
[[Category: Serial femtosecond crystallography]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: X-ray free-electron laser]]
+

Current revision

XFEL structure of the human delta opioid receptor in complex with a bifunctional peptide

PDB ID 4rwd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4rwd"
Personal tools