1z96

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:02, 14 February 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the Mud1 UBA domain==
==Crystal structure of the Mud1 UBA domain==
-
<StructureSection load='1z96' size='340' side='right' caption='[[1z96]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+
<StructureSection load='1z96' size='340' side='right'caption='[[1z96]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[1z96]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z96 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[1z96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z96 FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mud1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB], [http://www.ebi.ac.uk/pdbsum/1z96 PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [https://pdbe.org/1z96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB], [https://www.ebi.ac.uk/pdbsum/1z96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z96 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/MUD1_SCHPO MUD1_SCHPO]
-
The ubiquitin-pathway associated (UBA) domain is a 40-residue polyubiquitin-binding motif. The Schizosaccharomyces pombe protein Mud1 is an ortholog of the Saccharomyces cerevisiae DNA-damage response protein Ddi1 and binds to K48-linked polyubiquitin through its UBA domain. We have solved the crystal structure of Mud1 UBA at 1.8 angstroms resolution, revealing a canonical three-helical UBA fold. We have probed the interactions of this domain using mutagenesis, surface plasmon resonance, NMR and analytical ultracentrifugation. We show that the ubiquitin-binding surface of Mud1 UBA extends beyond previously recognized motifs and can be functionally dissected into primary and secondary ubiquitin-binding sites. Mutation of Phe330 to alanine, a residue exposed between helices 2 and 3, significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyubiquitin, despite leaving the primary binding surface functionally intact. Moreover, K48-linked diubiquitin binds a single Mud1 UBA domain even in the presence of excess UBA. We therefore propose a mechanism for the recognition of K48-linked polyubiquitin chains by Mud1 in which diubiquitin units are specifically recognized by a single UBA domain.
+
-
 
+
-
Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain.,Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:16138082<ref>PMID:16138082</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
-
[[Category: Brown, N R]]
+
[[Category: Brown NR]]
-
[[Category: Campbell, I D]]
+
[[Category: Campbell ID]]
-
[[Category: Endicott, J A]]
+
[[Category: Endicott JA]]
-
[[Category: Gordon, C]]
+
[[Category: Gordon C]]
-
[[Category: Johnson, L N]]
+
[[Category: Johnson LN]]
-
[[Category: Lowe, E D]]
+
[[Category: Lowe ED]]
-
[[Category: Noble, M E.M]]
+
[[Category: Noble MEM]]
-
[[Category: Trempe, J F]]
+
[[Category: Trempe J-F]]
-
[[Category: Protein transport]]
+
-
[[Category: Three-helix bundle]]
+
-
[[Category: Uba]]
+
-
[[Category: Ubiquitin]]
+

Current revision

Crystal structure of the Mud1 UBA domain

PDB ID 1z96

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z96"
Personal tools