2k48

Structural highlights

Function

NCAP_ANDV Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure (By similarity). As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini (By similarity). Involved in the transcription and replication initiation of vRNA by mediating primer annealing (By similarity). Plays a role in cap snatching by sequestering capped RNAs in P bodies for use by the viral RdRp during transcription initiation (By similarity). Substitutes for the cellular cap-binding complex (eIF4F) to preferentially facilitate the translation of capped mRNAs (By similarity). Initiates the translation by specifically binding to the cap and 40S ribosomal subunit (By similarity). Prevents the viral glycoprotein N (Gn) from autophagy-dependent breakdown maybe by blocking autophagosome formation (By similarity). Inhibits host EIF2AK2/PKR dimerization to prevent PKR-induced translational shutdown in cells and thus the activation of the antiviral state (PubMed:25410857). Inhibits IFN signaling responses directed by the dsRNA sensors RIGI and IFIH1/MDA5, probably by interacting with host E3 ubiquitin ligase TRIM21 (PubMed:24549848). As a consequence, TBK1-directed IRF3 phosphorylation and TBK1 autophosphorylation are inhibited (PubMed:24549848, PubMed:30867297). Also displays sequence-unspecific DNA endonuclease activity (By similarity).[UniProtKB:P05133][UniProtKB:Q89462]^{[1] [2] [3]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Nucleoprotein 3D structures

References

1. ↑ Cimica V, Dalrymple NA, Roth E, Nasonov A, Mackow ER. An innate immunity-regulating virulence determinant is uniquely encoded by the Andes virus nucleocapsid protein. mBio. 2014 Feb 18;5(1):e01088-13. PMID:24549848 doi:10.1128/mBio.01088-13
2. ↑ Wang Z, Mir MA. Andes virus nucleocapsid protein interrupts protein kinase R dimerization to counteract host interference in viral protein synthesis. J Virol. 2015 Feb;89(3):1628-39. PMID:25410857 doi:10.1128/JVI.02347-14
3. ↑ Simons MJ, Gorbunova EE, Mackow ER. Unique Interferon Pathway Regulation by the Andes Virus Nucleocapsid Protein Is Conferred by Phosphorylation of Serine 386. J Virol. 2019 May 1;93(10):e00338-19. PMID:30867297 doi:10.1128/JVI.00338-19
4. ↑ Wang Y, Boudreaux DM, Estrada DF, Egan CW, St Jeor SC, De Guzman RN. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem. 2008 Oct 17;283(42):28297-304. Epub 2008 Aug 7. PMID:18687679 doi:10.1074/jbc.M804869200