2ntc

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[[Image:2ntc.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of sv40 large T antigen origin binding domain with DNA==
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|PDB= 2ntc |SIZE=350|CAPTION= <scene name='initialview01'>2ntc</scene>, resolution 2.40&Aring;
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<StructureSection load='2ntc' size='340' side='right'caption='[[2ntc]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2ntc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian Virus 40])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntc OCA], [https://pdbe.org/2ntc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntc RCSB], [https://www.ebi.ac.uk/pdbsum/2ntc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntc ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q98ZP7_SV40 Q98ZP7_SV40]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ntc ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of sv40 large T antigen origin binding domain with DNA'''
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==See Also==
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*[[Large T Antigen|Large T Antigen]]
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__TOC__
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==Overview==
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</StructureSection>
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DNA replication is initiated upon binding of "initiators" to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV40 large T-antigen (T-ag) both with and without a subfragment of origin-containing DNA. In the co-structure, two T-ag obds are oriented in a head-to-head fashion on the same face of the DNA, and each T-ag obd engages the major groove. Although the obds are very close to each other when bound to this DNA target, they do not contact one another. These data provide a high-resolution structural model that explains site-specific binding to the origin and suggests how these interactions help direct the oligomerization events that culminate in assembly of the helicase-active dodecameric complex of T-ag.
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[[Category: Large Structures]]
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[[Category: Macaca mulatta polyomavirus 1]]
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==About this Structure==
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[[Category: Bohm A]]
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2NTC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTC OCA].
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[[Category: Bullock PA]]
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[[Category: Meinke G]]
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==Reference==
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The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA., Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A, PLoS Biol. 2007 Feb;5(2):e23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17253903 17253903]
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[[Category: Simian virus 40]]
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[[Category: Single protein]]
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[[Category: Bohm, A.]]
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[[Category: Bullock, P A.]]
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[[Category: Meinke, G.]]
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[[Category: origin binding domain; protein-dna complex; replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:50:52 2008''
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Current revision

Crystal Structure of sv40 large T antigen origin binding domain with DNA

PDB ID 2ntc

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