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2vdy

From Proteopedia

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Crystal structure of the reactive loop cleaved Corticosteroid Binding Globulin complexed with Cortisol

Structural highlights

2vdy is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

CBG_HUMAN Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry.

Function

CBG_HUMAN Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Corticosteroids are transported in the blood by a serpin, corticosteroid-binding globulin (CBG), and their normally equilibrated release can be further triggered by the cleavage of the reactive loop of CBG. We report here the crystal structures of cleaved human CBG (cCBG) at 1.8-A resolution and its complex with cortisol at 2.3-A resolution. As expected, on cleavage, CBG undergoes the irreversible S-to-R serpin transition, with the cleaved reactive loops being fully incorporated into the central beta-sheet. A connecting loop of helix D, which is in a helix-like conformation in native CBG, unwinds and grossly perturbs the hormone binding site following beta-sheet expansion in the cCBG structure but shifts away from the binding site by more than 8 A following the binding of cortisol. Unexpectedly, on cortisol binding, the hormone binding site of cCBG adopts a configuration almost identical with that of the native conformer. We conclude that CBG has adapted an allosteric mechanism of the serpins to allow equilibrated release of the hormones by a flip-flop movement of the intact reactive loop into and out of the beta-sheet. The change in the hormone binding affinity results from a change in the flexibility or plasticity of the connecting loop, which modulates the configuration of the binding site.

The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release.,Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW. The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release. J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745 doi:10.1016/j.jmb.2008.05.012
↑ Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW. The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release. J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745 doi:10.1016/j.jmb.2008.05.012

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vdy"

Categories: Homo sapiens | Large Structures | Read RJ | Wei Z | Zhou A

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE REACTIVE LOOP CLEAVED CORTICOSTEROID BINDING GLOBULIN COMPLEXED WITH CORTISOL==
-<StructureSection load='2vdy' size='340' side='right' caption='[[2vdy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+==Crystal structure of the reactive loop cleaved Corticosteroid Binding Globulin complexed with Cortisol==
+<StructureSection load='2vdy' size='340' side='right'caption='[[2vdy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vdy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VDY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vdy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VDY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HCY:(11ALPHA,14BETA)-11,17,21-TRIHYDROXYPREGN-4-ENE-3,20-DIONE'>HCY</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vdx|2vdx]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HCY:(11ALPHA,14BETA)-11,17,21-TRIHYDROXYPREGN-4-ENE-3,20-DIONE'>HCY</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vdy RCSB], [http://www.ebi.ac.uk/pdbsum/2vdy PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdy OCA], [https://pdbe.org/2vdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vdy RCSB], [https://www.ebi.ac.uk/pdbsum/2vdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vdy ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN]] Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN] Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN]] Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.<ref>PMID:18513745</ref>
+[https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN] Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.<ref>PMID:18513745</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vd/2vdy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vd/2vdy_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vdy ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 29: / Line 30: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2vdy" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 37: / Line 39: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Read, R J]]
+[[Category: Large Structures]]
-[[Category: Wei, Z]]
+[[Category: Read RJ]]
-[[Category: Zhou, A]]
+[[Category: Wei Z]]
-[[Category: Cbg]]
+[[Category: Zhou A]]
-[[Category: Cleaved]]
-[[Category: Corticosteroid binding globulin]]
-[[Category: Cortisol]]
-[[Category: Disease mutation]]
-[[Category: Glycoprotein]]
-[[Category: Lipid-binding]]
-[[Category: Secreted]]
-[[Category: Serpin]]
-[[Category: Steroid-binding]]
-[[Category: Transport]]
-[[Category: Transport protein]]

2vdy

From Proteopedia

Current revision

Crystal structure of the reactive loop cleaved Corticosteroid Binding Globulin complexed with Cortisol

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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