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2khf

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Plantaricin J in DPC-micelles

Structural highlights

2khf is a 1 chain structure with sequence from Lactiplantibacillus plantarum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P71461_LACPN

Publication Abstract from PubMed

The three-dimensional structures of the two peptides, PlnJ and PlnK, that constitutes the two-peptide bacteriocin plantaricin JK have been solved in water/TFE and water/DPC-micellar solutions using nuclear magnetic resonance (NMR) spectroscopy. PlnJ, a 25 residue peptide, has an N-terminal amphiphilic alpha-helix between Trp-3 and Tyr-15. The 32 residues long PlnK forms a central amphiphilic alpha-helix between Gly-9 and Leu-24. Measurements of the effect on anti-microbial activity of single glycine replacements in PlnJ and PlnK show that Gly-13 and Gly-17 in both peptides are very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. In variants where other glycine residues, Gly-20 in PlnJ and Gly-7, Gly-9, Gly-24 and Gly-25 in PlnK, were replaced, the activity was reduced less than 10-fold. It is proposed that the detrimental effect on activity when exchanging Gly-13 and Gly-17 in PlnJ and PlnK is a result of reduced ability of the two peptides to interact through the GxxxG-motifs constituting Gly-13 and Gly-17.

Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.,Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE Peptides. 2009 Sep;30(9):1613-21. Epub 2009 Jun 16. PMID:19538999^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE. Three-dimensional structure of the two-peptide bacteriocin plantaricin JK. Peptides. 2009 Sep;30(9):1613-21. Epub 2009 Jun 16. PMID:19538999 doi:10.1016/j.peptides.2009.06.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2khf"

@@ Line 1: / Line 1: @@
 ==Plantaricin J in DPC-micelles==
-<StructureSection load='2khf' size='340' side='right' caption='[[2khf]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2khf' size='340' side='right'caption='[[2khf]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2khf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KHF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2khf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KHF FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">plnJ, lp_0406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1590 Lactobacillus plantarum])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2khf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2khf RCSB], [http://www.ebi.ac.uk/pdbsum/2khf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2khf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khf OCA], [https://pdbe.org/2khf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2khf RCSB], [https://www.ebi.ac.uk/pdbsum/2khf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2khf ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/P71461_LACPN P71461_LACPN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 14: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2khf" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lactobacillus plantarum]]
+[[Category: Lactiplantibacillus plantarum]]
-[[Category: Haugen, C]]
+[[Category: Large Structures]]
-[[Category: Kristiansen, P E]]
+[[Category: Haugen C]]
-[[Category: Nissen-Meyer, J]]
+[[Category: Kristiansen PE]]
-[[Category: Rogne, P]]
+[[Category: Nissen-Meyer J]]
-[[Category: Anti-microbial]]
+[[Category: Rogne P]]
-[[Category: Antimicrobial protein]]
-[[Category: Bacteriocin]]
-[[Category: Two-peptide]]

2khf

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Current revision

Plantaricin J in DPC-micelles

Structural highlights

Function

Publication Abstract from PubMed

References

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