3fgq

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of native human neuroserpin

Structural highlights

3fgq is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.09Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

NEUS_HUMAN Familial encephalopathy with neuroserpin inclusion bodies. The disease is caused by mutations affecting the gene represented in this entry.

Function

NEUS_HUMAN Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Neuroserpin is a selective inhibitor of tissue-type plasminogen activator (tPA) that plays an important role in neuronal plasticity, memory, and learning. We report here the crystal structure of native human neuroserpin at 2.1 A resolution. The structure has a helical reactive center loop and an omega loop between strands 1B and 2B. The omega loop contributes to the inhibition of tPA, as deletion of this motif reduced the association rate constant with tPA by threefold but had no effect on the kinetics of interaction with urokinase. Point mutations in neuroserpin cause the formation of ordered intracellular polymers that underlie dementia familial encephalopathy with neuroserpin inclusion bodies (FENIB). Wild-type neuroserpin is also unstable and readily forms polymers under near-physiological conditions in vitro. This is, in part, due to the substitution of a conserved alanine for serine at position 340. The replacement of Ser340 by Ala increased the melting temperature by 3 degrees C and reduced polymerization as compared to wild-type neuroserpin. Similarly, neuroserpin has Asn-Leu-Val at the end of helix F and thus differs markedly from the Gly-X-Ile consensus sequence of the serpins. Restoration of these amino acids to the consensus sequence increased thermal stability and reduced the polymerization of neuroserpin and its transition to the latent conformer. Moreover, introduction of the consensus sequence into S49P neuroserpin that causes FENIB increased the stability and inhibitory activity of the mutant, as well as blocked polymerization and increased the yield of protein during refolding. These data provide a molecular explanation for the inherent instability of neuroserpin and the effect of point mutations that underlie the dementia FENIB.

The 2.1-A crystal structure of native neuroserpin reveals unique structural elements that contribute to conformational instability.,Takehara S, Onda M, Zhang J, Nishiyama M, Yang X, Mikami B, Lomas DA J Mol Biol. 2009 Apr 24;388(1):11-20. Epub 2009 Mar 10. PMID:19285087^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Takehara S, Onda M, Zhang J, Nishiyama M, Yang X, Mikami B, Lomas DA. The 2.1-A crystal structure of native neuroserpin reveals unique structural elements that contribute to conformational instability. J Mol Biol. 2009 Apr 24;388(1):11-20. Epub 2009 Mar 10. PMID:19285087 doi:http://dx.doi.org/10.1016/j.jmb.2009.03.007

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fgq"

@@ Line 1: / Line 1: @@
 ==Crystal structure of native human neuroserpin==
-<StructureSection load='3fgq' size='340' side='right' caption='[[3fgq]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
+<StructureSection load='3fgq' size='340' side='right'caption='[[3fgq]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3fgq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FGQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3fgq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FGQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fgq RCSB], [http://www.ebi.ac.uk/pdbsum/3fgq PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgq OCA], [https://pdbe.org/3fgq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fgq RCSB], [https://www.ebi.ac.uk/pdbsum/3fgq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgq ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/NEUS_HUMAN NEUS_HUMAN]] Familial encephalopathy with neuroserpin inclusion bodies. The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/NEUS_HUMAN NEUS_HUMAN] Familial encephalopathy with neuroserpin inclusion bodies. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/NEUS_HUMAN NEUS_HUMAN]] Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
+[https://www.uniprot.org/uniprot/NEUS_HUMAN NEUS_HUMAN] Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgq_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fgq ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 28: / Line 30: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3fgq" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Serpin|Serpin]]
+*[[Serpin 3D structures|Serpin 3D structures]]
 == References ==
 <references/>
@@ Line 36: / Line 39: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Mikami, B]]
+[[Category: Large Structures]]
-[[Category: Onda, M]]
+[[Category: Mikami B]]
-[[Category: Takehara, S]]
+[[Category: Onda M]]
-[[Category: Yang, X]]
+[[Category: Takehara S]]
-[[Category: Dementia]]
+[[Category: Yang X]]
-[[Category: Disease mutation]]
-[[Category: Glycoprotein]]
-[[Category: Hydrolase inhibitor]]
-[[Category: Inhibitor]]
-[[Category: Polymerization]]
-[[Category: Protease inhibitor]]
-[[Category: Secreted]]
-[[Category: Serine protease inhibitor]]
-[[Category: Serpin]]
-[[Category: Tpa]]

3fgq

From Proteopedia

Current revision

Crystal structure of native human neuroserpin

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox