3hht

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==A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability==
==A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability==
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<StructureSection load='3hht' size='340' side='right' caption='[[3hht]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
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<StructureSection load='3hht' size='340' side='right'caption='[[3hht]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3hht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeribacillus_pallidus Aeribacillus pallidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HHT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3hht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeribacillus_pallidus Aeribacillus pallidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HHT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CO:COBALT+(III)+ION'>3CO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CO:COBALT+(III)+ION'>3CO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hht OCA], [https://pdbe.org/3hht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hht RCSB], [https://www.ebi.ac.uk/pdbsum/3hht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hht ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hht RCSB], [http://www.ebi.ac.uk/pdbsum/3hht PDBsum]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q84FS5_9BACI Q84FS5_9BACI]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/3hht_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/3hht_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hht ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
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</StructureSection>
</StructureSection>
[[Category: Aeribacillus pallidus]]
[[Category: Aeribacillus pallidus]]
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[[Category: Nitrile hydratase]]
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[[Category: Large Structures]]
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[[Category: Bishop, A O.Tastan]]
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[[Category: Cowan DA]]
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[[Category: Cowan, D A]]
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[[Category: Sayed MF]]
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[[Category: Sayed, M F]]
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[[Category: Sewell BT]]
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[[Category: Sewell, B T]]
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[[Category: Tastan Bishop AO]]
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[[Category: Tsekoa, T L]]
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[[Category: Tsekoa TL]]
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[[Category: Wyk, J C.Van]]
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[[Category: Van Wyk JC]]
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[[Category: Lyase]]
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Current revision

A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability

PDB ID 3hht

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