2ost

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[[Image:2ost.gif|left|200px]]
 
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{{Structure
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==The structure of a bacterial homing endonuclease : I-Ssp6803I==
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|PDB= 2ost |SIZE=350|CAPTION= <scene name='initialview01'>2ost</scene>, resolution 3.100&Aring;
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<StructureSection load='2ost' size='340' side='right'caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OST FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [https://pdbe.org/2ost PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [https://www.ebi.ac.uk/pdbsum/2ost PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ost ProSAT]</span></td></tr>
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</table>
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'''The structure of a bacterial homing endonuclease : I-Ssp6803I'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q57253_SYNY3 Q57253_SYNY3]
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__TOC__
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==Overview==
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</StructureSection>
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The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
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[[Category: Large Structures]]
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[[Category: Synechocystis sp. PCC 6803]]
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==About this Structure==
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[[Category: Bonocora RP]]
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2OST is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA].
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[[Category: Shub DA]]
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[[Category: Stoddard BL]]
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==Reference==
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[[Category: Zhao L]]
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The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif., Zhao L, Bonocora RP, Shub DA, Stoddard BL, EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17410205 17410205]
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[[Category: Single protein]]
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[[Category: Synechocystis sp.]]
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[[Category: Bonocora, R P.]]
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[[Category: Shub, D A.]]
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[[Category: Stoddard, B L.]]
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[[Category: Zhao, L.]]
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[[Category: CA]]
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[[Category: group i intron]]
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[[Category: homing endonuclease]]
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[[Category: pd-(d/e)-xk motif]]
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[[Category: protein-dna complex]]
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[[Category: restriction enzyme fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:03:59 2008''
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Current revision

The structure of a bacterial homing endonuclease : I-Ssp6803I

PDB ID 2ost

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