This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ost

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:05, 21 February 2024) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
 +
==The structure of a bacterial homing endonuclease : I-Ssp6803I==
==The structure of a bacterial homing endonuclease : I-Ssp6803I==
-
<StructureSection load='2ost' size='340' side='right' caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+
<StructureSection load='2ost' size='340' side='right'caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OST FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OST FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [http://www.ebi.ac.uk/pdbsum/2ost PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [https://pdbe.org/2ost PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [https://www.ebi.ac.uk/pdbsum/2ost PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ost ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q57253_SYNY3 Q57253_SYNY3]
-
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
+
-
 
+
-
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif.,Zhao L, Bonocora RP, Shub DA, Stoddard BL EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205<ref>PMID:17410205</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Synechocystis sp]]
+
[[Category: Large Structures]]
-
[[Category: Bonocora, R P]]
+
[[Category: Synechocystis sp. PCC 6803]]
-
[[Category: Shub, D A]]
+
[[Category: Bonocora RP]]
-
[[Category: Stoddard, B L]]
+
[[Category: Shub DA]]
-
[[Category: Zhao, L]]
+
[[Category: Stoddard BL]]
-
[[Category: Group i intron]]
+
[[Category: Zhao L]]
-
[[Category: Homing endonuclease]]
+
-
[[Category: Hydrolase-dna complex]]
+
-
[[Category: Protein-dna complex]]
+
-
[[Category: Restriction enzyme fold]]
+

Current revision

The structure of a bacterial homing endonuclease : I-Ssp6803I

PDB ID 2ost

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ost"
Personal tools