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2an7

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Solution structure of the bacterial antidote ParD

Structural highlights

2an7 is a 2 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PARD_ECOLX Antitoxin component of a toxin-antitoxin (TA) module involved in plasmid partition. Inhibits the anti-DNA gyrase activity of toxin ParE; reverses and restores gyrase catalytic activity in vitro. The parDE operon alone is capable of stabilizing an RK2-derived minireplicon under defined growth conditions in several different Gram-negative bacteria. It does so by the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Binds its own promoter, autorepressing it; gentically only ParD is required for full autorepression.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE. These modules rely on differential stabilities of a highly expressed but labile antidote and a stable toxin expressed from one operon. Consequently, loss of the coding plasmid results in loss of the protective antidote and poisoning of the cell. The antidote protein usually also exhibits an autoregulatory function of the operon. In this paper, we present the solution structure of ParD. The repressor activity of ParD is mediated by the N-terminal half of the protein, which adopts a ribbon-helix-helix (RHH) fold. The C-terminal half of the protein is unstructured in the absence of its cognate binding partner ParE. Based on homology with other RHH proteins, we present a model of the ParD-DNA interaction, with the antiparallel beta-strand being inserted into the major groove of DNA. The fusion of the N-terminal DNA-binding RHH motif to the toxin-binding unstructured C-terminal domain is discussed in its evolutionary context.

The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding.,Oberer M, Zangger K, Gruber K, Keller W Protein Sci. 2007 Aug;16(8):1676-88. PMID:17656583^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jiang Y, Pogliano J, Helinski DR, Konieczny I. ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase. Mol Microbiol. 2002 May;44(4):971-9. PMID:12010492
↑ Roberts RC, Helinski DR. Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2. J Bacteriol. 1992 Dec;174(24):8119-32. PMID:1459960
↑ Roberts RC, Strom AR, Helinski DR. The parDE operon of the broad-host-range plasmid RK2 specifies growth inhibition associated with plasmid loss. J Mol Biol. 1994 Mar 18;237(1):35-51. PMID:8133518 doi:http://dx.doi.org/10.1006/jmbi.1994.1207
↑ Johnson EP, Strom AR, Helinski DR. Plasmid RK2 toxin protein ParE: purification and interaction with the ParD antitoxin protein. J Bacteriol. 1996 Mar;178(5):1420-9. PMID:8631720
↑ Oberer M, Zangger K, Gruber K, Keller W. The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding. Protein Sci. 2007 Aug;16(8):1676-88. PMID:17656583 doi:16/8/1676

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2an7"

@@ Line 1: / Line 1: @@
 ==Solution structure of the bacterial antidote ParD==
-<StructureSection load='2an7' size='340' side='right' caption='[[2an7]], [[NMR_Ensembles_of_Models | 24 NMR models]]' scene=''>
+<StructureSection load='2an7' size='340' side='right'caption='[[2an7]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2an7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AN7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2an7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AN7 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2an7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2an7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2an7 RCSB], [http://www.ebi.ac.uk/pdbsum/2an7 PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2an7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2an7 OCA], [https://pdbe.org/2an7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2an7 RCSB], [https://www.ebi.ac.uk/pdbsum/2an7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2an7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PARD_ECOLX PARD_ECOLX] Antitoxin component of a toxin-antitoxin (TA) module involved in plasmid partition. Inhibits the anti-DNA gyrase activity of toxin ParE; reverses and restores gyrase catalytic activity in vitro. The parDE operon alone is capable of stabilizing an RK2-derived minireplicon under defined growth conditions in several different Gram-negative bacteria. It does so by the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Binds its own promoter, autorepressing it; gentically only ParD is required for full autorepression.<ref>PMID:12010492</ref> <ref>PMID:1459960</ref> <ref>PMID:8133518</ref> <ref>PMID:8631720</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 13: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2an7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 18: / Line 23: @@
 </StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Gruber, K]]
+[[Category: Large Structures]]
-[[Category: Keller, W]]
+[[Category: Gruber K]]
-[[Category: Oberer, M]]
+[[Category: Keller W]]
-[[Category: Zangger, K]]
+[[Category: Oberer M]]
-[[Category: Bacterial antidote]]
+[[Category: Zangger K]]
-[[Category: Dna binding protein]]
-[[Category: Dna-binding motif]]
-[[Category: Plasmid addiction]]
-[[Category: Ribbon-helix-helix]]

2an7

From Proteopedia

Current revision

Solution structure of the bacterial antidote ParD

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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