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| | ==Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp with dT7== | | ==Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp with dT7== |
| - | <StructureSection load='2lxj' size='340' side='right' caption='[[2lxj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lxj' size='340' side='right'caption='[[2lxj]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lxj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ochroconis_lascauxensis Ochroconis lascauxensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LXJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LXJ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lxk|2lxk]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cspLA, cspA, cspL, lmo1364 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=994323 Ochroconis lascauxensis])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxj OCA], [https://pdbe.org/2lxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxj RCSB], [https://www.ebi.ac.uk/pdbsum/2lxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxj ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lxj RCSB], [http://www.ebi.ac.uk/pdbsum/2lxj PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CSPA_LISMO CSPA_LISMO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2lxj" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ochroconis lascauxensis]] | + | [[Category: Large Structures]] |
| - | [[Category: Jeong, K]] | + | [[Category: Listeria monocytogenes EGD-e]] |
| - | [[Category: Kim, Y]] | + | [[Category: Jeong K]] |
| - | [[Category: Lee, J]] | + | [[Category: Kim Y]] |
| - | [[Category: Nucleic acid]] | + | [[Category: Lee J]] |
| - | [[Category: Protein]]
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| - | [[Category: Transcription]]
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| Structural highlights
Function
CSPA_LISMO
Publication Abstract from PubMed
Cold-shock proteins (Csps), proteins expressed when the ambient temperature drops below the growth-supporting temperature, bind to single-stranded nucleic acids and act as RNA chaperones to regulate translation. Listeria monocytogenes is a psychrophilic food-borne pathogen that is problematic for the food industry. Structures of Csps from psychrophilic bacteria have not yet been studied. Despite dramatic differences in the thermostability of Csps of various thermophilic microorganisms, these proteins share a high degree of primary sequence homology and a high degree of three-dimensional structural similarity. Here, we investigated the structural and dynamic features as well as the thermostability of L. monocytogenes CspA (Lm-CspA). Lm-CspA has a five-stranded beta-barrel structure with hydrophobic core packing and two salt bridges. When heptathymidine (dT(7)) binds, values for the heteronuclear nuclear Overhauser effect and order parameters of residues in surface loop regions near nucleic acid binding sites increase dramatically. Moreover, Carr-Purcell-Meiboom-Gill experiments showed that slow motions observed for the nucleic acid binding residues K7, W8, F15, F27, and R56 disappeared in Lm-CspA-dT(7). Lm-CspA is less thermostable than mesophilic and thermophilic Csps, with a lower melting temperature (40 degrees C). The structural flexibility that accompanies longer surface loops and less hydrophobic core packing and a number of salt bridges and unfavorable electrostatic repulsion are likely key factors in the low thermostability of Lm-CspA. This implies that the large conformational flexibility of psychrophilic Lm-CspA, which more easily accommodates nucleic acids at low temperature, is required for RNA chaperone function under cold-shock conditions and for the cold adaptation of L. monocytogenes.
Structural and dynamic features of cold-shock proteins of Listeria monocytogenes, a psychrophilic bacterium.,Lee J, Jeong KW, Jin B, Ryu KS, Kim EH, Ahn JH, Kim Y Biochemistry. 2013 Apr 9;52(14):2492-504. doi: 10.1021/bi301641b. Epub 2013 Apr, 1. PMID:23506337[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee J, Jeong KW, Jin B, Ryu KS, Kim EH, Ahn JH, Kim Y. Structural and dynamic features of cold-shock proteins of Listeria monocytogenes, a psychrophilic bacterium. Biochemistry. 2013 Apr 9;52(14):2492-504. doi: 10.1021/bi301641b. Epub 2013 Apr, 1. PMID:23506337 doi:10.1021/bi301641b
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