2p8w

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[[Image:2p8w.jpg|left|200px]]
 
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{{Structure
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==Fitted structure of eEF2 in the 80S:eEF2:GDPNP cryo-EM reconstruction==
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|PDB= 2p8w |SIZE=350|CAPTION= <scene name='initialview01'>2p8w</scene>
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<SX load='2p8w' size='340' side='right' viewer='molstar' caption='[[2p8w]], [[Resolution|resolution]] 11.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene>
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<table><tr><td colspan='2'>[[2p8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P8W FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p8w OCA], [https://pdbe.org/2p8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p8w RCSB], [https://www.ebi.ac.uk/pdbsum/2p8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p8w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EF2_YEAST EF2_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p8/2p8w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p8w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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On the basis of kinetic data on ribosome protein synthesis, the mechanical energy for translocation of the mRNA-tRNA complex is thought to be provided by GTP hydrolysis of an elongation factor (eEF2 in eukaryotes, EF-G in bacteria). We have obtained cryo-EM reconstructions of eukaryotic ribosomes complexed with ADP-ribosylated eEF2 (ADPR-eEF2), before and after GTP hydrolysis, providing a structural basis for analyzing the GTPase-coupled mechanism of translocation. Using the ADP-ribosyl group as a distinct marker, we observe conformational changes of ADPR-eEF2 that are due strictly to GTP hydrolysis. These movements are likely representative of native eEF2 motions in a physiological context and are sufficient to uncouple the mRNA-tRNA complex from two universally conserved bases in the ribosomal decoding center (A1492 and A1493 in Escherichia coli) during translocation. Interpretation of these data provides a detailed two-step model of translocation that begins with the eEF2/EF-G binding-induced ratcheting motion of the small ribosomal subunit. GTP hydrolysis then uncouples the mRNA-tRNA complex from the decoding center so translocation of the mRNA-tRNA moiety may be completed by a head rotation of the small subunit.
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'''Fitted structure of eEF2 in the 80S:eEF2:GDPNP cryo-EM reconstruction'''
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Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation.,Taylor DJ, Nilsson J, Merrill AR, Andersen GR, Nissen P, Frank J EMBO J. 2007 May 2;26(9):2421-31. Epub 2007 Apr 19. PMID:17446867<ref>PMID:17446867</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2p8w" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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On the basis of kinetic data on ribosome protein synthesis, the mechanical energy for translocation of the mRNA-tRNA complex is thought to be provided by GTP hydrolysis of an elongation factor (eEF2 in eukaryotes, EF-G in bacteria). We have obtained cryo-EM reconstructions of eukaryotic ribosomes complexed with ADP-ribosylated eEF2 (ADPR-eEF2), before and after GTP hydrolysis, providing a structural basis for analyzing the GTPase-coupled mechanism of translocation. Using the ADP-ribosyl group as a distinct marker, we observe conformational changes of ADPR-eEF2 that are due strictly to GTP hydrolysis. These movements are likely representative of native eEF2 motions in a physiological context and are sufficient to uncouple the mRNA-tRNA complex from two universally conserved bases in the ribosomal decoding center (A1492 and A1493 in Escherichia coli) during translocation. Interpretation of these data provides a detailed two-step model of translocation that begins with the eEF2/EF-G binding-induced ratcheting motion of the small ribosomal subunit. GTP hydrolysis then uncouples the mRNA-tRNA complex from the decoding center so translocation of the mRNA-tRNA moiety may be completed by a head rotation of the small subunit.
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2P8W is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P8W OCA].
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__TOC__
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</SX>
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==Reference==
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[[Category: Large Structures]]
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Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation., Taylor DJ, Nilsson J, Merrill AR, Andersen GR, Nissen P, Frank J, EMBO J. 2007 May 2;26(9):2421-31. Epub 2007 Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17446867 17446867]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Andersen, G R.]]
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[[Category: Andersen GR]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Merrill, A R.]]
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[[Category: Merrill AR]]
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[[Category: Nilsson, J.]]
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[[Category: Nilsson J]]
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[[Category: Nissen, P.]]
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[[Category: Nissen P]]
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[[Category: Taylor, D J.]]
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[[Category: Taylor DJ]]
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[[Category: GNP]]
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[[Category: 80s ribosome]]
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[[Category: elongation]]
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[[Category: gtpase]]
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[[Category: translocation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:10:15 2008''
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Current revision

Fitted structure of eEF2 in the 80S:eEF2:GDPNP cryo-EM reconstruction

2p8w, resolution 11.30Å

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