2w89

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the E.coli tRNAArg aminoacyl stem issoacceptor RR-1660 at 2.0 Angstroem resolution

Structural highlights

2w89 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Due to the redundancy of the genetic code there exist six mRNA codons for arginine and several tRNA(Arg) isoacceptors which translate these triplets to protein within the context of the mRNA. The tRNA identity elements assure the correct aminoacylation of the tRNA with the cognate amino acid by the aminoacyl-tRNA-synthetases. In tRNA(Arg), the identity elements consist of the anticodon, parts of the D-loop and the discriminator base. The minor groove of the acceptor stem interacts with the arginyl-tRNA-synthetase. We crystallized different Escherichia coli tRNA(Arg) acceptor stem helices and solved the structure of the tRNA(Arg) isoacceptor RR-1660 microhelix by X-ray structure analysis. The acceptor stem helix crystallizes in the space group P1 with the cell constants a=26.28, b=28.92, c=29.00 A, alpha=105.74, beta=99.01, gamma=97.44 degrees and two molecules per asymmetric unit. The RNA hydration pattern consists of 88 bound water molecules. Additionally, one glycerol molecule is bound within the interface of the two RNA molecules.

Crystal structure of the E. coli tRNA(Arg) aminoacyl stem isoacceptor RR-1660 at 2.0 A resolution.,Eichert A, Perbandt M, Oberthur D, Schreiber A, Furste JP, Betzel C, Erdmann VA, Forster C Biochem Biophys Res Commun. 2009 Jul 17;385(1):84-7. Epub 2009 May 6. PMID:19426710^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Eichert A, Perbandt M, Oberthur D, Schreiber A, Furste JP, Betzel C, Erdmann VA, Forster C. Crystal structure of the E. coli tRNA(Arg) aminoacyl stem isoacceptor RR-1660 at 2.0 A resolution. Biochem Biophys Res Commun. 2009 Jul 17;385(1):84-7. Epub 2009 May 6. PMID:19426710 doi:10.1016/j.bbrc.2009.04.158

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w89"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the E.coli tRNAArg aminoacyl stem issoacceptor RR-1660 at 2.0 Angstroem resolution==
-<StructureSection load='2w89' size='340' side='right' caption='[[2w89]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='2w89' size='340' side='right'caption='[[2w89]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w89]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W89 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w89]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W89 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w89 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w89 RCSB], [http://www.ebi.ac.uk/pdbsum/2w89 PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w89 OCA], [https://pdbe.org/2w89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w89 RCSB], [https://www.ebi.ac.uk/pdbsum/2w89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w89 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 14: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2w89" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Betzel, C]]
+[[Category: Escherichia coli]]
-[[Category: Eichert, A]]
+[[Category: Large Structures]]
-[[Category: Erdmann, V A]]
+[[Category: Betzel C]]
-[[Category: Foerster, C]]
+[[Category: Eichert A]]
-[[Category: Fuerste, J P]]
+[[Category: Erdmann VA]]
-[[Category: Perbandt, M]]
+[[Category: Foerster C]]
-[[Category: Schreiber, A]]
+[[Category: Fuerste JP]]
-[[Category: Hydration]]
+[[Category: Perbandt M]]
-[[Category: Rna]]
+[[Category: Schreiber A]]
-[[Category: Trna acceptor stem]]
-[[Category: Trna identity]]
-[[Category: Trnaarg]]

2w89

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Current revision

Crystal structure of the E.coli tRNAArg aminoacyl stem issoacceptor RR-1660 at 2.0 Angstroem resolution

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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