2pc6

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[[Image:2pc6.gif|left|200px]]
 
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{{Structure
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==Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea==
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|PDB= 2pc6 |SIZE=350|CAPTION= <scene name='initialview01'>2pc6</scene>, resolution 2.50&Aring;
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<StructureSection load='2pc6' size='340' side='right'caption='[[2pc6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=SIN:SUCCINIC ACID'>SIN</scene>
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<table><tr><td colspan='2'>[[2pc6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Niteu Niteu]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2fgd 2fgd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PC6 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6]
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
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|GENE= ilvH, NE1324 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718])
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fgd|2fgd]], [[2fgc|2fgc]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ilvH, NE1324 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 NITEU])</td></tr>
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'''Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea'''
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lyase Lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pc6 OCA], [https://pdbe.org/2pc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pc6 RCSB], [https://www.ebi.ac.uk/pdbsum/2pc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pc6 ProSAT], [https://www.topsan.org/Proteins/MCSG/2pc6 TOPSAN]</span></td></tr>
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</table>
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==Overview==
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/2pc6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pc6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.
Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.
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==About this Structure==
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Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.,Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W Protein Sci. 2007 Jul;16(7):1360-7. PMID:17586771<ref>PMID:17586771</ref>
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2PC6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718]. This structure supersedes the now removed PDB entry 2FGD. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PC6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit., Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W, Protein Sci. 2007 Jul;16(7):1360-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17586771 17586771]
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</div>
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<div class="pdbe-citations 2pc6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lyase]]
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[[Category: Niteu]]
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[[Category: Chruszcz, M]]
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[[Category: Cymborowski, M T]]
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[[Category: Edwards, A]]
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[[Category: Joachimiak, A]]
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[[Category: Structural genomic]]
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[[Category: Minor, W]]
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[[Category: Onopriyenko, O]]
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[[Category: Petkowski, J J]]
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[[Category: Savchenko, A]]
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[[Category: Skarina, T]]
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[[Category: Zheng, H]]
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[[Category: Zimmerman, M D]]
[[Category: Acetolactate synthase]]
[[Category: Acetolactate synthase]]
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[[Category: Nitrosomonas europaea atcc 19718]]
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[[Category: Mcsg]]
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[[Category: Single protein]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Chruszcz, M.]]
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[[Category: Regulatory subunit]]
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[[Category: Cymborowski, M T.]]
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[[Category: Edwards, A.]]
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[[Category: Joachimiak, A.]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Minor, W.]]
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[[Category: Onopriyenko, O.]]
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[[Category: Petkowski, J J.]]
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[[Category: Savchenko, A.]]
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[[Category: Skarina, T.]]
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[[Category: Zheng, H.]]
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[[Category: Zimmerman, M D.]]
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[[Category: CA]]
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[[Category: SIN]]
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[[Category: acetolactate synthase]]
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[[Category: lyase]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: regulatory subunit]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:11:25 2008''
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Current revision

Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea

PDB ID 2pc6

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