4xvc
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4xvc is ON HOLD Authors: Yuzhong Zhang, Pingyi Li Description: Crystal structure of an esterase from the bacterial hormone-sensitive lipase (HSL) f...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of an esterase from the bacterial hormone-sensitive lipase (HSL) family== | |
| + | <StructureSection load='4xvc' size='340' side='right'caption='[[4xvc]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4xvc]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Environmental_samples Environmental samples]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XVC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xvc OCA], [https://pdbe.org/4xvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xvc RCSB], [https://www.ebi.ac.uk/pdbsum/4xvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xvc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microbial hormone-sensitive lipases (HSLs) contain a CAP domain and a catalytic domain. However, it remains unclear how the CAP domain interacts with the catalytic domain to maintain the stability of microbial HSLs. Here, we isolated an HSL esterase, E40, from a marine sedimental metagenomic library. E40 exhibited the maximal activity at 45 degrees C, and was quite thermolabile, with a half life of only 2 min at 40 degrees C, which may be an adaptation of E40 to the permanently cold sediment environment. The structure of E40 was solved to study its thermolability. Structural analysis showed that E40 lacks the inter-domain hydrophobic interactions between the loop1 of the CAP domain and the alpha7 of the catalytic domain compared to its thermostable homologs. Mutational analysis showed that, the introduction of hydrophobic residues Trp202 and Phe203 in the alpha7 significantly improved E40 stability, and that a further introduction of hydrophobic residues in the loop1 made E40 more thermostable due to the formation of inter-domain hydrophobic interactions. Altogether, the results indicate that the absence of inter-domain hydrophobic interactions between the loop1 and the alpha7 leads to the thermolability of E40. In addition, a comparative analysis of the structures of E40 and other thermolabile and thermostable HSLs suggests that the inter-domain hydrophobic interactions between the loop1 and the alpha7 are a key element for the thermostability of microbial HSLs. Therefore, this study not only illustrates the structural element leading to the thermolability of E40, but also reveals a structural determinant for HSL thermostability. | ||
| - | + | Inter-Domain Hydrophobic Interactions Modulate the Thermostability of Microbial Esterases from the Hormone-Sensitive Lipase Family.,Li PY, Chen XL, Ji P, Li CY, Wang P, Zhang Y, Xie BB, Qin QL, Su HN, Zhou BC, Zhang YZ, Zhang XY J Biol Chem. 2015 Mar 14. pii: jbc.M115.646182. PMID:25771540<ref>PMID:25771540</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4xvc" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Environmental samples]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Li P]] | ||
| + | [[Category: Zhang Y]] | ||
Current revision
Crystal structure of an esterase from the bacterial hormone-sensitive lipase (HSL) family
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