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2mru

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==Structure of truncated EcMazE-DNA complex==
==Structure of truncated EcMazE-DNA complex==
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<StructureSection load='2mru' size='340' side='right' caption='[[2mru]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>
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<StructureSection load='2mru' size='340' side='right'caption='[[2mru]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mru]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MRU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mru]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MRU FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2mrn|2mrn]], [[1mvf|1mvf]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mru OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mru RCSB], [http://www.ebi.ac.uk/pdbsum/2mru PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mru OCA], [https://pdbe.org/2mru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mru RCSB], [https://www.ebi.ac.uk/pdbsum/2mru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mru ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MAZE_ECOLI MAZE_ECOLI]] Antitoxin component of a toxin-antitoxin (TA) module. Labile antitoxin that binds to the MazF mRNA interferase toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell will die in its absence. The endoribonuclease activity (MazF, a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium the overexpression of MazF leads to programmed cell death. Cell growth and viability are not affected when MazF and MazE are coexpressed. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription. Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation, while MazE-MazF is also implicated in cell death in liquid media.
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[https://www.uniprot.org/uniprot/MAZE_ECOLI MAZE_ECOLI] Antitoxin component of a toxin-antitoxin (TA) module. Labile antitoxin that binds to the MazF mRNA interferase toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell will die in its absence. The endoribonuclease activity (MazF, a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium the overexpression of MazF leads to programmed cell death. Cell growth and viability are not affected when MazF and MazE are coexpressed. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription. Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation, while MazE-MazF is also implicated in cell death in liquid media.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 2mru" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Buts, L]]
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[[Category: Escherichia coli K-12]]
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[[Category: Loris, R]]
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[[Category: Large Structures]]
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[[Category: Nuland, N van]]
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[[Category: Buts L]]
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[[Category: Zorzini, V]]
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[[Category: Loris R]]
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[[Category: Antitoxin]]
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[[Category: Zorzini V]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Van Nuland N]]
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[[Category: Dna-binding domain]]
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[[Category: Maze]]
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[[Category: Protein-dna complex]]
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[[Category: Transcription]]
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Current revision

Structure of truncated EcMazE-DNA complex

PDB ID 2mru

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