4wem

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==Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1==
==Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1==
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<StructureSection load='4wem' size='340' side='right' caption='[[4wem]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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<StructureSection load='4wem' size='340' side='right'caption='[[4wem]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4wem]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4wem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WEM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hlr|3hlr]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wem RCSB], [http://www.ebi.ac.uk/pdbsum/4wem PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wem OCA], [https://pdbe.org/4wem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wem RCSB], [https://www.ebi.ac.uk/pdbsum/4wem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wem ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FAEG2_ECOLX FAEG2_ECOLX] K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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Oral feed-based passive immunization can be a promising strategy to prolong maternal lactogenic immunity against postweaning infections. Enterotoxigenic Escherichia coli (ETEC)-caused postweaning diarrhea in piglets is one such infection that may be prevented by oral passive immunization and might avert recurrent economic losses to the pig farming industry. As a proof of principle, we designed anti-ETEC antibodies by fusing variable domains of llama heavy chain-only antibodies (VHHs) against ETEC to the Fc part of a porcine immunoglobulin (IgG or IgA) and expressed them in Arabidopsis thaliana seeds. In this way, four VHH-IgG and four VHH-IgA antibodies were produced to levels of about 3% and 0.2% of seed weight, respectively. Cotransformation of VHH-IgA with the porcine joining chain and secretory component led to the production of light-chain devoid, assembled multivalent dimeric, and secretory IgA-like antibodies. In vitro analysis of all of the antibody-producing seed extracts showed inhibition of bacterial binding to porcine gut villous enterocytes. However, in the piglet feed-challenge experiment, only the piglets receiving feed containing the VHH-IgA-based antibodies (dose 20 mg/d per pig) were protected. Piglets receiving the VHH-IgA-based antibodies in the feed showed a progressive decline in shedding of bacteria, significantly lower immune responses corroborating reduced exposure to the ETEC pathogen, and a significantly higher weight gain compared with the piglets receiving VHH-IgG producing (dose 80 mg/d per pig) or wild-type seeds. These results stress the importance of the antibody format in oral passive immunization and encourage future expression of these antibodies in crop seeds.
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Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding.
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Orally fed seeds producing designer IgAs protect weaned piglets against enterotoxigenic Escherichia coli infection.,Virdi V, Coddens A, De Buck S, Millet S, Goddeeris BM, Cox E, De Greve H, Depicker A Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11809-14. doi:, 10.1073/pnas.1301975110. Epub 2013 Jun 25. PMID:23801763<ref>PMID:23801763</ref>
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Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.,Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907<ref>PMID:25828907</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 4wem" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Pilin 3D structures|Pilin 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Broeck, I Van den]]
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[[Category: Escherichia coli]]
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[[Category: Greve, H De]]
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[[Category: Lama glama]]
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[[Category: Kerpel, M De]]
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[[Category: Large Structures]]
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[[Category: Moonens, K]]
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[[Category: De Greve H]]
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[[Category: Pardon, E]]
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[[Category: De Kerpel M]]
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[[Category: Remaut, H]]
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[[Category: Moonens K]]
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[[Category: Adhesin]]
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[[Category: Pardon E]]
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[[Category: Complex]]
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[[Category: Remaut H]]
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[[Category: Llama single domain antibody]]
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[[Category: Van den Broeck I]]
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[[Category: Nanobody]]
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[[Category: Structural protein]]
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Current revision

Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1

PDB ID 4wem

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