4xxj
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4xxj is ON HOLD Authors: Bratanov, D., Balandin, T., Round, E., Gushchin, I., Gordeliy, V. Description: Category: Unreleased Structures [[Cate...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Escherichia coli-Expressed Halobacterium salinarum Bacteriorhodopsin in the Trimeric Form== | |
| + | <StructureSection load='4xxj' size='340' side='right'caption='[[4xxj]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4xxj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XXJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xxj OCA], [https://pdbe.org/4xxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xxj RCSB], [https://www.ebi.ac.uk/pdbsum/4xxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xxj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allows one to localize the underlying problem of poor expression of a membrane protein using bR as an example. Our approach is based on constructing chimeric proteins comprising parts of a protein of interest and complementary parts of a homologous protein demonstrating advantageous expression. This complementary protein approach allowed us to increase bR expression by two orders of magnitude through the introduction of two silent mutations into bR coding DNA. For the first time the high quality crystals of bR expressed in E. Coli were obtained using the produced protein. The crystals obtained with in meso nanovolume crystallization diffracted to 1.67 A. | ||
| - | + | An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin.,Bratanov D, Balandin T, Round E, Shevchenko V, Gushchin I, Polovinkin V, Borshchevskiy V, Gordeliy V PLoS One. 2015 Jun 5;10(6):e0128390. doi: 10.1371/journal.pone.0128390., eCollection 2015. PMID:26046789<ref>PMID:26046789</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4xxj" style="background-color:#fffaf0;"></div> |
| - | [[Category: Balandin | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]] |
| - | [[Category: Round | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Halobacterium salinarum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Balandin T]] | ||
| + | [[Category: Bratanov D]] | ||
| + | [[Category: Gordeliy V]] | ||
| + | [[Category: Gushchin I]] | ||
| + | [[Category: Round E]] | ||
Current revision
Crystal Structure of Escherichia coli-Expressed Halobacterium salinarum Bacteriorhodopsin in the Trimeric Form
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