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2pq3

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N-Terminal Calmodulin Zn-Trapped Intermediate

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OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pq3"

Categories: Large Structures | Rattus norvegicus | Guo Q | Tang WJ | Warren JT

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-[[Image:2pq3.gif|left|200px]]
- {{Structure
+==N-Terminal Calmodulin Zn-Trapped Intermediate==
-|PDB= 2pq3 |SIZE=350|CAPTION= <scene name='initialview01'>2pq3</scene>, resolution 1.30&Aring;
+<StructureSection load='2pq3' size='340' side='right'caption='[[2pq3]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+<table><tr><td colspan='2'>[[2pq3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PQ3 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-|GENE= Calm1, Calm, Cam, Cam1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pq3 OCA], [https://pdbe.org/2pq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pq3 RCSB], [https://www.ebi.ac.uk/pdbsum/2pq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pq3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CALM1_RAT CALM1_RAT] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB:P62158]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pq/2pq3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pq3 ConSurf].
+<div style="clear:both"></div>
-'''N-Terminal Calmodulin Zn-Trapped Intermediate'''
+==See Also==
+*[[Calmodulin 3D structures|Calmodulin 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Calmodulin (CaM) is a 16.8-kDa calcium-binding protein involved in calcium-signal transduction. It is the canonical member of the EF-hand family of proteins, which are characterized by a helix-loop-helix calcium-binding motif. CaM is composed of N- and C-terminal globular domains (N-CaM and C-CaM), and within each domain there are two EF-hand motifs. Upon binding calcium, CaM undergoes a significant, global conformational change involving reorientation of the four helix bundles in each of its two domains. This conformational change upon ion binding is a key component of the signal transduction and regulatory roles of CaM, yet the precise nature of this transition is still unclear. Here, we present a 1.3-A structure of zinc-bound N-terminal calmodulin (N-CaM) solved by single-wavelength anomalous diffraction phasing of a selenomethionyl N-CaM. Our zinc-bound N-CaM structure differs from previously reported CaM structures and resembles calcium-free apo-calmodulin (apo-CaM), despite the zinc binding to both EF-hand motifs. Structural comparison with calcium-free apo-CaM, calcium-loaded CaM, and a cross-linked calcium-loaded CaM suggests that our zinc-bound N-CaM reveals an intermediate step in the initiation of metal ion binding at the first EF-hand motif. Our data also suggest that metal ion coordination by two key residues in the first metal-binding site represents an initial step in the conformational transition induced by metal binding. This is followed by reordering of the N-terminal region of the helix exiting from this first binding loop. This conformational switch should be incorporated into models of either stepwise conformational transition or flexible, dynamic energetic state sampling-based transition.
+[[Category: Large Structures]]
-==About this Structure==
-PQ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQ3 OCA].
-==Reference==
-A 1.3-A structure of zinc-bound N-terminal domain of calmodulin elucidates potential early ion-binding step., Warren JT, Guo Q, Tang WJ, J Mol Biol. 2007 Nov 23;374(2):517-27. Epub 2007 Sep 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17942116 17942116]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Guo Q]]
-[[Category: Guo, Q.]]
+[[Category: Tang WJ]]
-[[Category: Tang, W J.]]
+[[Category: Warren JT]]
-[[Category: Warren, J T.]]
-[[Category: CAC]]
-[[Category: ZN]]
-[[Category: calmodulin]]
-[[Category: cam]]
-[[Category: ef-hand]]
-[[Category: helix-turn-helix]]
-[[Category: metal binding protein]]
-[[Category: n-cam]]
-[[Category: n-terminal calmodulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:16:04 2008''

2pq3

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N-Terminal Calmodulin Zn-Trapped Intermediate

Structural highlights

Function

Evolutionary Conservation

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