4elt

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==Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing modified pyrimidines==
==Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing modified pyrimidines==
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<StructureSection load='4elt' size='340' side='right' caption='[[4elt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='4elt' size='340' side='right'caption='[[4elt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4elt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ELT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4elt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ELT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0R5:2-DEOXY-5-[(4-ETHYNYLPHENYL)ETHYNYL]URIDINE+5-(TETRAHYDROGEN+TRIPHOSPHATE)'>0R5</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0R5:2-DEOXY-5-[(4-ETHYNYLPHENYL)ETHYNYL]URIDINE+5-(TETRAHYDROGEN+TRIPHOSPHATE)'>0R5</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ojs|3ojs]], [[3oju|3oju]], [[4elu|4elu]], [[4elv|4elv]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4elt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elt OCA], [https://pdbe.org/4elt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4elt RCSB], [https://www.ebi.ac.uk/pdbsum/4elt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4elt ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">polA, pol1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4elt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4elt RCSB], [http://www.ebi.ac.uk/pdbsum/4elt PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/DPO1_THEAQ DPO1_THEAQ]
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Modified nucleotides play a paramount role in many cutting-edge biomolecular techniques. The present structural study highlights the plasticity and flexibility of the active site of a DNA polymerase while incorporating non-polar "Click-able" nucleotide analogs and emphasizes new insights into rational design guidelines for modified nucleotides.
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Interactions of non-polar and "Click-able" nucleotides in the confines of a DNA polymerase active site.,Obeid S, Busskamp H, Welte W, Diederichs K, Marx A Chem Commun (Camb). 2012 Aug 28;48(67):8320-2. Epub 2012 Jul 6. PMID:22766607<ref>PMID:22766607</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[DNA polymerase|DNA polymerase]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Diederichs, K]]
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[[Category: Diederichs K]]
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[[Category: Marx, A]]
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[[Category: Marx A]]
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[[Category: Obeid, S]]
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[[Category: Obeid S]]
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[[Category: A family]]
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[[Category: Dna polymerase]]
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[[Category: Dna synthesis]]
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[[Category: Modified nucleotide]]
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[[Category: Non-natural nucleotide]]
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[[Category: Rigid linker]]
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[[Category: Transferase-dna complex]]
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Current revision

Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing modified pyrimidines

PDB ID 4elt

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