4y0w
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4y0w is ON HOLD Authors: Milani, M. Description: YeaZ from Pseudomonas aeruginosa Category: Unreleased Structures Category: Milani, M) |
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| - | '''Unreleased structure''' | ||
| - | + | ==YeaZ from Pseudomonas aeruginosa== | |
| + | <StructureSection load='4y0w' size='340' side='right'caption='[[4y0w]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4y0w]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO579 Pseudomonas aeruginosa PAO579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0W FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0w OCA], [https://pdbe.org/4y0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0w RCSB], [https://www.ebi.ac.uk/pdbsum/4y0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A140UHA7_PSEAI A0A140UHA7_PSEAI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Pseudomonas aeruginosa PA3685 locus encodes a conserved protein that shares 49% sequence identity with Escherichiacoli YeaZ, which was recently reported as involved in the biosynthesis of threonylcarbamoyl adenosine (t6A), a universal modified tRNA nucleoside. Many YeaZ orthologues were reported as "essential for life" among various bacterial species, suggesting a critical role for both these proteins and for the t6A biosynthetic pathway. We provide here evidences that PA3685 protein (PaYeaZ) is essential. Additionally, we describe its purification, crystallization, and crystallographic structure. The crystal structure shows that PaYeaZ is composed of two domains one of which is the platform to form protein-protein interaction involved either in homodimeric assembly or in the formation of the multiprotein complex required for the synthesis of t6A. These features make the PaYeaZ protein a potential target candidate for the design of novel inhibitors able to hinder the complex formation and expected to abolish the crucial activity of t6A synthesis. | ||
| - | + | Crystal structure of YeaZ from Pseudomonas aeruginosa.,Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G Biochem Biophys Res Commun. 2016 Jan 6. pii: S0006-291X(16)30008-0. doi:, 10.1016/j.bbrc.2016.01.008. PMID:26768361<ref>PMID:26768361</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Milani | + | <div class="pdbe-citations 4y0w" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO579]] | ||
| + | [[Category: Milani M]] | ||
Current revision
YeaZ from Pseudomonas aeruginosa
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