4q25

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==Crystal structure of PhoU from Pseudomonas aeruginosa==
==Crystal structure of PhoU from Pseudomonas aeruginosa==
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<StructureSection load='4q25' size='340' side='right' caption='[[4q25]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
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<StructureSection load='4q25' size='340' side='right'caption='[[4q25]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4q25]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q25 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4q25]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q25 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q25 RCSB], [http://www.ebi.ac.uk/pdbsum/4q25 PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q25 OCA], [https://pdbe.org/4q25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q25 RCSB], [https://www.ebi.ac.uk/pdbsum/4q25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q25 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/PHOU_PSEAE PHOU_PSEAE]] Plays a role in the regulation of phosphate uptake.
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[https://www.uniprot.org/uniprot/PHOU_PSEAE PHOU_PSEAE] Plays a role in the regulation of phosphate uptake.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Escherichia coli, seven genes (pstS, pstC, pstA, pstB, phoU, phoR, and phoB) are involved in sensing environmental phosphate (Pi) and controlling the expression of the Pho regulon. PhoU is a negative regulator of the Pi-signaling pathway and modulates Pi transport through Pi transporter proteins (PstS, PstC, PstA, and PstB) through the two-component system PhoR and PhoB. Inactivation of PhoY2, one of the two PhoU homologs in Mycobacterium tuberculosis, causes defects in persistence phenotypes and increased susceptibility to antibiotics and stresses. Despite the important biological role, the mechanism of PhoU function is still unknown. Here we have determined the crystal structure of PhoU from Pseudomonas aeruginosa. It exists as a dimer in the crystal, with each monomer consisting of two structurally similar three-helix bundles. Our equilibrium sedimentation measurements support the reversible monomer-dimer equilibrium model in which P. aeruginosa PhoU exists in solution predominantly as dimers, with monomers in a minor fraction, at low protein concentrations. The dissociation constant for PhoU dimerization is 3.2x10(-6)M. The overall structure of P. aeruginosa PhoU dimer resembles those of Aquifex aeolicus PhoU and Thermotoga maritima PhoU2. However, it shows distinct structural features in some loops and the dimerization pattern.
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Crystal structure of PhoU from Pseudomonas aeruginosa, a negative regulator of the Pho regulon.,Lee SJ, Park YS, Kim SJ, Lee BJ, Suh SW J Struct Biol. 2014 Oct;188(1):22-9. doi: 10.1016/j.jsb.2014.08.010. Epub 2014, Sep 8. PMID:25220976<ref>PMID:25220976</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lee, B J]]
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[[Category: Large Structures]]
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[[Category: Lee, S J]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Suh, S W]]
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[[Category: Lee B-J]]
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[[Category: Helix bundle]]
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[[Category: Lee SJ]]
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[[Category: Membrane]]
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[[Category: Suh SW]]
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[[Category: Unknown function]]
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Current revision

Crystal structure of PhoU from Pseudomonas aeruginosa

PDB ID 4q25

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