Class C beta-lactamase

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Beta-lactamases are awesome
Beta-lactamases are awesome
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<Structure load='1ke4' size='400' frame='true' color='white' align='right' caption='AmpC Class C Beta-lactamase' />
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<Structure load='1ke4' size='350' frame='true' color='white' align='right' caption='E. coli AmpC Class C Beta-lactamase complex with phosphate (PDB code [[1ke4]])' />
== Background and beta-lactam antibiotics ==
== Background and beta-lactam antibiotics ==
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
 
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Since the discovery of penicillin in the late 1920s, β-lactam antibiotics, characterized by their central chemical structure, the β-lactam ring, have played an important role in human health (Fig 1). Unfortunately, extensive use, and often misuse, of such drugs has led to an increased resistance in many species of bacterium resulting in major clinical treatment dilemmas. Each year in the United States alone, a minimum of 2 million people are infected with drug-resistant bacteria and of those 2 million, at least 23,000 infections result in fatality.
 
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[[Image:Beta-lactam.jpg]]
 
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Clinically, β-lactam antibiotics are utilized to combat bacterial infections by targeting penicillin-binding proteins (PBPs). PBPs are enzymes that are located in the cell membrane and function in cross-linking to form the peptidoglycan layer. PBPs have a deprotonated serine which executes nucleophilic attack on the carbonyl carbon. The PBP is then covalently attached to one unit of peptidoglycan. The amino group of an alanine on a second unit of peptidoglycan then performs a second nucleophilic attack on the carbonyl carbon, resulting in two covalently cross-linked peptidoglycan units and the regeneration of the catalytic PBP (Fig 2).
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Since the discovery of penicillin in the late 1920s, β-lactam antibiotics, characterized by their central chemical structure, the β-lactam ring, have played an important role in human health (Fig 1). Unfortunately, extensive use, and often misuse, of such drugs has led to an increased resistance in many species of bacterium resulting in major clinical treatment dilemmas. Each year in the United States alone, a minimum of 2 million people are infected with drug-resistant bacteria and of those 2 million, at least 23,000 infections result in fatality.<ref>Antibiotic Resistant Threat Report in the United States, 2013. Centers for Disease Control and Prevention. 16 September, 2013.
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The β-lactam ring covalently attaches to PBPs, inhibiting them from executing their role in properly synthesizing the cell wall peptidoglycan layer, via nucleophilic attack of the carbonyl carbon (Fig 3). The β-lactam cannot be removed and thus permanently renders the PBP incapable of its catalytic function in cross-linking. Ultimately, this results in death of bacterial cells from osmotic instability or autolysis
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Current revision

Beta-lactamases are awesome

E. coli AmpC Class C Beta-lactamase complex with phosphate (PDB code 1ke4)

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Background and beta-lactam antibiotics

Since the discovery of penicillin in the late 1920s, β-lactam antibiotics, characterized by their central chemical structure, the β-lactam ring, have played an important role in human health (Fig 1). Unfortunately, extensive use, and often misuse, of such drugs has led to an increased resistance in many species of bacterium resulting in major clinical treatment dilemmas. Each year in the United States alone, a minimum of 2 million people are infected with drug-resistant bacteria and of those 2 million, at least 23,000 infections result in fatality.[1]

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Benjamin E. Nicholson, Michal Harel

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