4p94

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'''Unreleased structure'''
 
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The entry 4p94 is ON HOLD until Jul 11 2016
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==The crystal structure of the soluble domain of Sulfolobus acidocaldarius FlaF (residues 35-164)==
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<StructureSection load='4p94' size='340' side='right'caption='[[4p94]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4p94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P94 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.651&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p94 OCA], [https://pdbe.org/4p94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p94 RCSB], [https://www.ebi.ac.uk/pdbsum/4p94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p94 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q4J9K8_SULAC Q4J9K8_SULAC]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-A and 1.65-A resolution crystal structures of this monotopic membrane protein. Structures revealed an N-terminal alpha-helix and an eight-strand beta-sandwich, immunoglobulin-like fold with striking similarity to S-layer proteins. Crystal structures, X-ray scattering, and mutational analyses suggest dimer assembly is needed for in vivo function. The sole cell envelope component of S. acidocaldarius is a paracrystalline S-layer, and FlaF specifically bound to S-layer protein, suggesting that its interaction domain is located in the pseudoperiplasm with its N-terminal helix in the membrane. From these data, FlaF may act as the previously unknown archaellum stator protein that anchors the rotating archaellum to the archaeal cell envelope.
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Authors: Tsai, C.-L., Arvai, A.S., Ishida, J.P., Tainer, J.A.
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FlaF Is a beta-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein.,Banerjee A, Tsai CL, Chaudhury P, Tripp P, Arvai AS, Ishida JP, Tainer JA, Albers SV Structure. 2015 Apr 3. pii: S0969-2126(15)00079-9. doi:, 10.1016/j.str.2015.03.001. PMID:25865246<ref>PMID:25865246</ref>
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Description: The crystal structure of the soluble domain of Sulfolobus acidocaldarius FlaF (residues 35-164)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tsai, C.-L]]
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<div class="pdbe-citations 4p94" style="background-color:#fffaf0;"></div>
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[[Category: Arvai, A.S]]
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[[Category: Ishida, J.P]]
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==See Also==
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[[Category: Tainer, J.A]]
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*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sulfolobus acidocaldarius DSM 639]]
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[[Category: Arvai AS]]
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[[Category: Ishida JP]]
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[[Category: Tainer JA]]
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[[Category: Tsai C-L]]

Current revision

The crystal structure of the soluble domain of Sulfolobus acidocaldarius FlaF (residues 35-164)

PDB ID 4p94

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