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| | ==Hev b 6.02 (hevein) extracted from surgical gloves== | | ==Hev b 6.02 (hevein) extracted from surgical gloves== |
| - | <StructureSection load='4wp4' size='340' side='right' caption='[[4wp4]], [[Resolution|resolution]] 1.43Å' scene=''> | + | <StructureSection load='4wp4' size='340' side='right'caption='[[4wp4]], [[Resolution|resolution]] 1.43Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wp4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WP4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WP4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wp4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WP4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WP4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q9b|1q9b]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wp4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wp4 RCSB], [http://www.ebi.ac.uk/pdbsum/4wp4 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wp4 OCA], [https://pdbe.org/4wp4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wp4 RCSB], [https://www.ebi.ac.uk/pdbsum/4wp4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wp4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth. | + | [https://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4wp4" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Hevea brasiliensis]] | | [[Category: Hevea brasiliensis]] |
| - | [[Category: Galicia, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Rodriguez-Romero, A]] | + | [[Category: Galicia C]] |
| - | [[Category: Allergen]] | + | [[Category: Rodriguez-Romero A]] |
| - | [[Category: Hevea]]
| + | |
| - | [[Category: Lectin]]
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| - | [[Category: Rubber]]
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| - | [[Category: Surgical glove]]
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| Structural highlights
Function
HEVE_HEVBR N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
Publication Abstract from PubMed
Latex allergy is a health problem that mainly affects medical environments, causing anaphylactic shocks in extreme cases. Sensitization and reactions to this material is closely linked to the use of latex gloves. The objective of this study was to purify two of the major allergens from latex surgical gloves to study the biochemical and structural changes that could be generated during the product manufacture and to compare their IgE recognition with the non-processed allergens. Glycosylated allergen Hev b 2 (beta-1,3-glucanase) and Hev b 6.02 (hevein) were purified from glove extracts using affinity (Concanavalin A) and reversed-phase chromatographies, respectively. ELISA experiments were performed with both proteins and sera from allergic patients to assess the IgE recognition, which was heterogeneous. Crystallographic methods were used to obtain the 3D structure of Hev b 6.02 from surgical gloves, which did not show evident modification when compared with the protein from the natural non-processed form. Despite having the same crystallographic structure, the IgE from some patients showed different recognition when the glove and the natural allergen were used in ELISA. Furthermore, using electrophoretic techniques, we identified three forms of Hev b 2: one corresponding to the complete polypeptide chain with posttranslational modifications, and two glycosylated fragments. The mixture of these three forms showed stronger recognition by IgE from latex-allergic patients than the pure non-processed allergen. In conclusion, IgE from subjects sensitized to latex products showed different recognition between the allergens obtained from a natural source and the processed material, even when the structure was maintained. This demonstrates the importance of using processed allergens in further investigations of diagnosis, prevalence, product allergenicity, and therapies.
Impact of the vulcanization process on the structural characteristics and IgE recognition of two allergens, Hev b 2 and Hev b 6.02, extracted from latex surgical gloves.,Galicia C, Mendoza-Hernandez G, Rodriguez-Romero A Mol Immunol. 2015 Feb 17;65(2):250-258. doi: 10.1016/j.molimm.2015.01.018. PMID:25700348[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Galicia C, Mendoza-Hernandez G, Rodriguez-Romero A. Impact of the vulcanization process on the structural characteristics and IgE recognition of two allergens, Hev b 2 and Hev b 6.02, extracted from latex surgical gloves. Mol Immunol. 2015 Feb 17;65(2):250-258. doi: 10.1016/j.molimm.2015.01.018. PMID:25700348 doi:http://dx.doi.org/10.1016/j.molimm.2015.01.018
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