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| | ==Pyrobaculum calidifontis esterase monoclinic form== | | ==Pyrobaculum calidifontis esterase monoclinic form== |
| - | <StructureSection load='2yh2' size='340' side='right' caption='[[2yh2]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='2yh2' size='340' side='right'caption='[[2yh2]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2yh2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_21063 Dsm 21063]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YH2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2yh2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrobaculum_calidifontis Pyrobaculum calidifontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YH2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wir|2wir]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yh2 OCA], [https://pdbe.org/2yh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2yh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yh2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yh2 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8NKS0_9CREN Q8NKS0_9CREN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2yh2" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Carboxylesterase]] | + | [[Category: Large Structures]] |
| - | [[Category: Dsm 21063]] | + | [[Category: Pyrobaculum calidifontis]] |
| - | [[Category: Bogdanovic, X]] | + | [[Category: Bogdanovic X]] |
| - | [[Category: Hinrichs, W]] | + | [[Category: Hinrichs W]] |
| - | [[Category: Palm, G J]] | + | [[Category: Palm GJ]] |
| - | [[Category: Alpha/beta hydrolase fold]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Hyperthermophilic enzyme]]
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| - | [[Category: Tertiary alcohol]]
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| Structural highlights
Function
Q8NKS0_9CREN
Publication Abstract from PubMed
The highly thermostable esterase from the hyperthermophilic archaeon Pyrobaculum calidifontis VA1 (PestE) shows high enantioselectivity (E > 100) in the kinetic resolution of racemic chiral carboxylic acids, but little selectivity towards acetates of tertiary alcohols (E = 2-4). To explain these unique properties, its crystal structure has been determined at 2.0 A resolution. The enzyme is a member of the hormone-sensitive lipase group (group H) of the esterase/lipase superfamily on the basis of the amino acid sequence identity. The PestE structure shows a canonical alpha/beta-hydrolase fold as core domain with a cap structure at the C-terminal end of the beta-sheet. A tetramer in the crystal packing is formed of two dimers; the dimeric form is observed in solution. Conserved dimers and even tetramers are found in other group H proteins. The amino acid residues Ser157, His284, and Asp254 form the catalytic triad, which is typically found in alpha/beta-hydrolases. The oxyanion hole is composed of Gly85 and Gly86 within the conserved sequence motif HGGG(M,F,W) (amino acid residues 83-87) and Ala158. With the elucidated structure, experimental results about enantioselectivity towards the two model substrate classes (as exemplified for 3-phenylbutanoic acid ethyl ester and 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate) could be explained by molecular modeling. For both enantiomers of the tertiary alcohol, orientations in two binding pockets were obtained without significant energy differences corresponding to the observed low enantioselectivity due to missing steric repulsions. In contrast, for the carboxylic acid ester, two different orientations with significant energy differences for each enantiomer were found matching the high E values.
The crystal structure of an esterase from the hyperthermophilic microorganism Pyrobaculum calidifontis VA1 explains its enantioselectivity.,Palm GJ, Fernandez-Alvaro E, Bogdanovic X, Bartsch S, Sczodrok J, Singh RK, Bottcher D, Atomi H, Bornscheuer UT, Hinrichs W Appl Microbiol Biotechnol. 2011 Aug;91(4):1061-72. Epub 2011 May 26. PMID:21614503[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Palm GJ, Fernandez-Alvaro E, Bogdanovic X, Bartsch S, Sczodrok J, Singh RK, Bottcher D, Atomi H, Bornscheuer UT, Hinrichs W. The crystal structure of an esterase from the hyperthermophilic microorganism Pyrobaculum calidifontis VA1 explains its enantioselectivity. Appl Microbiol Biotechnol. 2011 Aug;91(4):1061-72. Epub 2011 May 26. PMID:21614503 doi:10.1007/s00253-011-3337-9
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