1exf

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[[Image:1exf.gif|left|200px]]<br />
 
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<applet load="1exf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1exf, resolution 2.1&Aring;" />
 
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'''EXFOLIATIVE TOXIN A'''<br />
 
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==Overview==
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==EXFOLIATIVE TOXIN A==
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Exfoliative toxin A (ETA) causes staphylococcal scalded skin syndrome, which is characterized by a specific intraepidermal separation of layers, of the skin. The mechanism by which ETA causes skin separation is unknown, although protease or superantigen activity has been implicated. The X-ray, crystal structure of ETA has been solved in two crystal forms to 2.1 and, 2.3 A resolution and R-factors of 17% and 19%, respectively. The, structures indicate that ETA belongs to the chymotrypsin-like family of, serine proteases and cleaves substrates after acidic residues. The, conformation of a loop adjacent to the catalytic site is suggested to be, key in regulating the proteolytic activity of ETA through controlling, whether the main chain carbonyl group of Pro192 occupies the oxyanion, hole. A unique amino-terminal domain containing a 15-residue amphipathic, alpha helix may also be involved in protease activation through binding a, specific receptor. Substitution of the active site serine residue with, cysteine abolishes the ability of ETA to produce the characteristic, separation of epidermal layers but not its ability to induce T cell, proliferation.
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<StructureSection load='1exf' size='340' side='right'caption='[[1exf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1exf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EXF FirstGlance]. <br>
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1EXF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] and [http://en.wikipedia.org/wiki/Unidentified Unidentified] with GLY as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: CAT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EXF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1exf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1exf OCA], [https://pdbe.org/1exf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1exf RCSB], [https://www.ebi.ac.uk/pdbsum/1exf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1exf ProSAT]</span></td></tr>
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The structure of the superantigen exfoliative toxin A suggests a novel regulation as a serine protease., Vath GM, Earhart CA, Rago JV, Kim MH, Bohach GA, Schlievert PM, Ohlendorf DH, Biochemistry. 1997 Feb 18;36(7):1559-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9048539 9048539]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/ETA_STAAU ETA_STAAU] Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS).<ref>PMID:2117445</ref> <ref>PMID:2384148</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1exf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1exf ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Unidentified]]
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[[Category: Bohach GA]]
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[[Category: Bohach, G.A.]]
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[[Category: Earhart CA]]
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[[Category: Earhart, C.A.]]
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[[Category: Kim MH]]
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[[Category: Kim, M.H.]]
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[[Category: Ohlendorf DH]]
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[[Category: Ohlendorf, D.H.]]
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[[Category: Rago JV]]
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[[Category: Rago, J.V.]]
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[[Category: Schlievert PM]]
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[[Category: Schlievert, P.M.]]
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[[Category: Vath GM]]
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[[Category: Vath, G.M.]]
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[[Category: GLY]]
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[[Category: complex (toxin/peptide)]]
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[[Category: hydrolase]]
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[[Category: serine protease]]
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[[Category: superantigen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:14:16 2007''
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Current revision

EXFOLIATIVE TOXIN A

PDB ID 1exf

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