4r3u
From Proteopedia
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==Crystal structure of 2-Hydroxyisobutyryl-CoA Mutase== | ==Crystal structure of 2-Hydroxyisobutyryl-CoA Mutase== | ||
| - | <StructureSection load='4r3u' size='340' side='right' caption='[[4r3u]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4r3u' size='340' side='right'caption='[[4r3u]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4r3u]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R3U OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[4r3u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquincola_tertiaricarbonis Aquincola tertiaricarbonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R3U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3HC:3-HYDROXYBUTANOYL-COENZYME+A'>3HC</scene>, <scene name='pdbligand=3KK:S-{(3R,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]-3,5,9-TRIHYDROXY-8,8-DIMETHYL-3,5-DIOXIDO-10,14-DIOXO-2,4,6-TRIOXA-11,15-DIAZA-3LAMBDA~5~,5LAMBDA~5~-DIPHOSPHAHEPTADECAN-17-YL}+2-HYDROXY-2-METHYLPROPANETHIOATE'>3KK</scene>, <scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3HC:3-HYDROXYBUTANOYL-COENZYME+A'>3HC</scene>, <scene name='pdbligand=3KK:S-{(3R,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]-3,5,9-TRIHYDROXY-8,8-DIMETHYL-3,5-DIOXIDO-10,14-DIOXO-2,4,6-TRIOXA-11,15-DIAZA-3LAMBDA~5~,5LAMBDA~5~-DIPHOSPHAHEPTADECAN-17-YL}+2-HYDROXY-2-METHYLPROPANETHIOATE'>3KK</scene>, <scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r3u OCA], [https://pdbe.org/4r3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r3u RCSB], [https://www.ebi.ac.uk/pdbsum/4r3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r3u ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HCMA_AQUTE HCMA_AQUTE] Together with HcmB, catalyzes the isomerization of 2-hydroxyisobutyryl-CoA and 3-hydroxybutyryl-CoA. Is specific for 2-hydroxyisobutyryl-CoA and (S)-3-hydroxybutyryl-CoA, and shows only very low activity with (R)-3-hydroxybutyryl-CoA, isobutyryl-CoA and butyryl-CoA (PubMed:22433853, PubMed:25720495). In vitro, can isomerize pivalyl-CoA and isovaleryl-CoA, with much lower efficiency (PubMed:25720495). Plays a central role in the degradation of substrates bearing a tert-butyl moiety, such as the fuel oxygenate methyl tert-butyl ether (MTBE) and its metabolites (PubMed:22433853).<ref>PMID:22433853</ref> <ref>PMID:25720495</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Aquincola tertiaricarbonis]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Kurteva-Yaneva N]] |
| - | [[Category: | + | [[Category: Rohwerder T]] |
| - | [[Category: | + | [[Category: Straeter N]] |
| - | [[Category: | + | [[Category: Zahn M]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of 2-Hydroxyisobutyryl-CoA Mutase
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