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4qxx

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==Structure of the amyloid forming peptide GNLVS (residues 26-30) from the eosinophil major basic protein (EMBP)==
==Structure of the amyloid forming peptide GNLVS (residues 26-30) from the eosinophil major basic protein (EMBP)==
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<StructureSection load='4qxx' size='340' side='right' caption='[[4qxx]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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<StructureSection load='4qxx' size='340' side='right'caption='[[4qxx]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4qxx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QXX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QXX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4qxx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QXX FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qxx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qxx RCSB], [http://www.ebi.ac.uk/pdbsum/4qxx PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.445&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qxx OCA], [https://pdbe.org/4qxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qxx RCSB], [https://www.ebi.ac.uk/pdbsum/4qxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qxx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/PRG2_HUMAN PRG2_HUMAN]] Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.<ref>PMID:10913121</ref>
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[https://www.uniprot.org/uniprot/PRG2_HUMAN PRG2_HUMAN] Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.<ref>PMID:10913121</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Eosinophils are white blood cells that function in innate immunity and participate in the pathogenesis of various inflammatory and neoplastic disorders. Their secretory granules contain four cytotoxic proteins, including the eosinophil major basic protein (MBP-1). How MBP-1 toxicity is controlled within the eosinophil itself and activated upon extracellular release is unknown. Here we show how intragranular MBP-1 nanocrystals restrain toxicity, enabling its safe storage, and characterize them with an X-ray-free electron laser. Following eosinophil activation, MBP-1 toxicity is triggered by granule acidification, followed by extracellular aggregation, which mediates the damage to pathogens and host cells. Larger non-toxic amyloid plaques are also present in tissues of eosinophilic patients in a feedback mechanism that likely limits tissue damage under pathological conditions of MBP-1 oversecretion. Our results suggest that MBP-1 aggregation is important for innate immunity and immunopathology mediated by eosinophils and clarify how its polymorphic self-association pathways regulate toxicity intra- and extracellularly.
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Toxicity of Eosinophil MBP Is Repressed by Intracellular Crystallization and Promoted by Extracellular Aggregation.,Soragni A, Yousefi S, Stoeckle C, Soriaga AB, Sawaya MR, Kozlowski E, Schmid I, Radonjic-Hoesli S, Boutet S, Williams GJ, Messerschmidt M, Seibert MM, Cascio D, Zatsepin NA, Burghammer M, Riekel C, Colletier JP, Riek R, Eisenberg DS, Simon HU Mol Cell. 2015 Feb 25. pii: S1097-2765(15)00049-0. doi:, 10.1016/j.molcel.2015.01.026. PMID:25728769<ref>PMID:25728769</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Eisenberg, D]]
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[[Category: Homo sapiens]]
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[[Category: Sawaya, M R]]
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[[Category: Large Structures]]
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[[Category: Soragni, A]]
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[[Category: Eisenberg D]]
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[[Category: Soriaga, A B]]
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[[Category: Sawaya MR]]
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[[Category: Amyloid-like protofibril]]
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[[Category: Soragni A]]
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[[Category: Protein fibril]]
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[[Category: Soriaga AB]]

Current revision

Structure of the amyloid forming peptide GNLVS (residues 26-30) from the eosinophil major basic protein (EMBP)

PDB ID 4qxx

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