5akq
From Proteopedia
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==X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC== | ==X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC== | ||
| - | <StructureSection load='5akq' size='340' side='right' caption='[[5akq]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='5akq' size='340' side='right'caption='[[5akq]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5akq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AKQ FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [https://pdbe.org/5akq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [https://www.ebi.ac.uk/pdbsum/5akq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD] Transforms N-isopropylammelide to cyanuric acid and isopropylamine. |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The N-isopropylammelide isopropylaminohydrolase from Pseudomonas sp. strain ADP, AtzC, provides the third hydrolytic step in the mineralization of s-triazine herbicides, such as atrazine. We obtained the X-ray crystal structure of AtzC at 1.84 A with a weak inhibitor bound in the active site and then used a combination of in silico docking and site-directed mutagenesis to understand the interactions between AtzC and its substrate, isopropylammelide. The substitution of an active site histidine residue (His249) for an alanine abolished the enzyme's catalytic activity. We propose a plausible catalytic mechanism, consistent with the biochemical and crystallographic data obtained that is similar to that found in carbonic anhydrase and other members of subtype III of the amidohydrolase family. | ||
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| + | X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC.,Balotra S, Warden AC, Newman J, Briggs LJ, Scott C, Peat TS PLoS One. 2015 Sep 21;10(9):e0137700. doi: 10.1371/journal.pone.0137700., eCollection 2015. PMID:26390431<ref>PMID:26390431</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5akq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Balotra | + | [[Category: Pseudomonas sp. ADP]] |
| - | [[Category: Briggs | + | [[Category: Balotra S]] |
| - | [[Category: Newman | + | [[Category: Briggs LJ]] |
| - | [[Category: Peat | + | [[Category: Newman J]] |
| - | [[Category: Scott | + | [[Category: Peat TS]] |
| - | [[Category: Warden | + | [[Category: Scott C]] |
| - | + | [[Category: Warden AC]] | |
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| - | + | ||
Current revision
X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC
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