4yp2
From Proteopedia
(Difference between revisions)
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Cleavage of nicotinamide adenine dinucleotides by the ribosome inactivating protein from Momordica charantia== | |
| + | <StructureSection load='4yp2' size='340' side='right'caption='[[4yp2]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4yp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YP2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yp2 OCA], [https://pdbe.org/4yp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yp2 RCSB], [https://www.ebi.ac.uk/pdbsum/4yp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yp2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RIP1_MOMCH RIP1_MOMCH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The interaction of momordin, a type 1 ribosome-inactivating protein from Momordica charantia, with NADP(+) and NADPH has been investigated by X-ray diffraction analysis of complexes generated by co-crystallization and crystal soaking. It is known that the proteins of this family readily cleave the adenine-ribose bond of adenosine and related nucleotides in the crystal, leaving the product, adenine, bound to the enzyme active site. Surprisingly, the nicotinamide-ribose bond of oxidized NADP(+) is cleaved, leaving nicotinamide bound in the active site in the same position but in a slightly different orientation to that of the five-membered ring of adenine. No binding or cleavage of NADPH was observed at pH 7.4 in these experiments. These observations are in accord with current views of the enzyme mechanism and may contribute to ongoing searches for effective inhibitors. | ||
| - | + | Cleavage of nicotinamide adenine dinucleotide by the ribosome-inactivating protein from Momordica charantia.,Vinkovic M, Dunn G, Wood GE, Husain J, Wood SP, Gill R Acta Crystallogr F Struct Biol Commun. 2015 Sep 1;71(Pt 9):1152-5. doi:, 10.1107/S2053230X15013540. Epub 2015 Aug 25. PMID:26323301<ref>PMID:26323301</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Gill | + | <div class="pdbe-citations 4yp2" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: Wood | + | *[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]] |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Momordica charantia]] | ||
| + | [[Category: Gill R]] | ||
| + | [[Category: Hussain J]] | ||
| + | [[Category: Vinkovic M]] | ||
| + | [[Category: Wood GE]] | ||
| + | [[Category: Wood SP]] | ||
Current revision
Cleavage of nicotinamide adenine dinucleotides by the ribosome inactivating protein from Momordica charantia
| |||||||||||
